Study 783

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Study information

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• Quality control
  • Retracted paper
  • Contamination issues suspected
  • Batch effect issues suspected
  • Uncontrolled confounding suspected
  • Results are suspect (various reasons)
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Needs review

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

Study 783

DOI Digital object identifier for electronic documents.

10.1128/spectrum.03796-22

URI

https://journals.asm.org/doi/10.1128/spectrum.03796-22

Authors

Journal

Year

Experiment 1

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incomplete

Curated date: 2024/03/11

Curator:

Revision editor(s):

Subjects

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Oral cavity Bucca,Buccal cavity,Cavity of mouth,Oral cavity,oral cavity

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Healthy Control

Group 1 name Corresponds to the case (exposed) group for case-control studies

Type 2 Diabetes Mellitus Patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Patients with fasting plasma glucose >= 7.0mmol/L, HbA1C ≥6.5%, and 2-h postprandial blood glucose ≥11.1 mmol/L

Group 0 sample size Number of subjects in the control (unexposed) group

10

Group 1 sample size Number of subjects in the case (exposed) group

10

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

Participants were excluded if they had used antibiotics in the past 6 months, or local antibiotics in the past 7 days

Lab analysis

Sequencing type

WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

MGISEQ-2000

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

ANOSIM

LEfSe

PLS-DA (Partial least square discriminant analysis)

Spearman Correlation

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes