Exploring the gut microbiota in patients with pre-diabetes and treatment naïve diabetes type 2 - a pilot study
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Norway
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- type II diabetes mellitus adult onset diabetes,Adult-Onset Diabetes,adult-onset diabetes,Adult-Onset Diabetes Mellitus,diabetes mellitis type 2,diabetes mellitis type II,DIABETES MELLITUS TYPE 02,diabetes mellitus type 2,Diabetes Mellitus, Adult Onset,Diabetes Mellitus, Adult-Onset,Diabetes Mellitus, Ketosis Resistant,Diabetes Mellitus, Ketosis-Resistant,Diabetes Mellitus, Maturity Onset,Diabetes Mellitus, Maturity-Onset,Diabetes Mellitus, Non Insulin Dependent,Diabetes Mellitus, Non-Insulin-Dependent,Diabetes Mellitus, Noninsulin Dependent,diabetes mellitus, noninsulin-dependent,Diabetes Mellitus, Slow Onset,Diabetes Mellitus, Slow-Onset,Diabetes Mellitus, Stable,Diabetes Mellitus, Type 2,diabetes mellitus, type 2,diabetes mellitus, type 2, protection against,Diabetes Mellitus, Type II,Diabetes, Type 2,diabetes, type 2,insulin resistance, susceptibility to,Ketosis-Resistant Diabetes Mellitus,Maturity Onset Diabetes Mellitus,maturity-onset diabetes,Maturity-Onset Diabetes Mellitus,MODY,NIDDM,Non-Insulin Dependent Diabetes,non-insulin dependent diabetes,Non-Insulin Dependent Diabetes Mellitus,non-insulin dependent diabetes mellitus,non-insulin-dependent diabetes mellitus,noninsulin dependent diabetes,noninsulin-dependent diabetes mellitus,Slow-Onset Diabetes Mellitus,Stable Diabetes Mellitus,T2DM - Type 2 Diabetes mellitus,T2DM - type 2 diabetes mellitus,Type 2 Diabetes,type 2 diabetes,Type 2 Diabetes Mellitus,type 2 diabetes mellitus,Type 2 Diabetes Mellitus Non-Insulin Dependent,type 2 diabetes mellitus non-insulin dependent,Type II Diabetes,type II diabetes,type II diabetes mellitus
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Pre Type 2 Diabetes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with pre type 2 diabetes
- Group 0 sample size Number of subjects in the control (unexposed) group
- 48
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 4 weeks
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V9
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, body mass index, sex, Confounders controlled for: "f-cal" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.f-cal
Signature 1
Source: Table 3
Description: Significant differential microbial abundance between groups of people with pre T2D and healthy groups
Abundance in Group 1: increased abundance in Pre Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Bacilli | ||
Eisenbergiella | ||
Eisenbergiella massiliensis | ||
Lactobacillales | ||
Streptococcaceae | ||
Streptococcus | ||
Neglectibacter timonensis | ||
Neglectibacter |
Revision editor(s): Kadeniyi
Signature 2
Source: Table 3
Description: Significant differential microbial abundance between groups of people with pre-T2D and healthy groups
Abundance in Group 1: decreased abundance in Pre Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Oscillibacter valericigenes | ||
Phocaeicola coprocola CAG:162 |
Revision editor(s): Kadeniyi
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Treatment naive Type 2 Diabetes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- A group of adult patients diagnosed with Type 2 Diabetes.
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex, body mass index, Confounders controlled for: "F-cal" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.F-cal
Signature 1
Source: Table 3
Description: Significant differential microbial abundance between groups of people with T2D and healthy groups
Abundance in Group 1: increased abundance in Treatment naive Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Dorea | ||
Dorea formicigenerans | ||
Dorea longicatena | ||
Erysipelotrichaceae | ||
Erysipelotrichia | ||
Erysipelotrichales | ||
Turicibacter | ||
Turicibacter sanguinis |
Revision editor(s): Kadeniyi
Signature 2
Source: Table 3
Description: Significant differential microbial abundance between groups of people with T2D and healthy groups
Abundance in Group 1: decreased abundance in Treatment naive Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Anaerotignum |
Revision editor(s): Kadeniyi
Experiment 3
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Pre Type 2 Diabetes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with pre type 2 diabetes
- Group 0 sample size Number of subjects in the control (unexposed) group
- 38
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V9
Statistical Analysis
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, body mass index, sex, Confounders controlled for: "F-cal" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.F-cal
Signature 1
Source: Table 3
Description: Significant differential microbial abundance between groups of people with pre T2D and healthy groups
Abundance in Group 1: increased abundance in Pre Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Dorea | ||
Pseudomonadota | ||
Enterobacterales | ||
Shigella | ||
Escherichia |
Revision editor(s): Kadeniyi
Signature 2
Source: Table 3
Description: Significant differential microbial abundance between groups of people with pre T2D and healthy groups
Abundance in Group 1: decreased abundance in Pre Type 2 Diabetes
NCBI | Quality Control | Links |
---|---|---|
Roseburia | ||
Roseburia intestinalis | ||
Dialister invisus | ||
Veillonella | ||
Faecalibacterium prausnitzii | ||
Sutterella wadsworthensis |
Revision editor(s): Kadeniyi