Shotgun sequencing of the vaginal microbiome reveals both a species and functional potential signature of preterm birth/Experiment 3
From BugSigDB
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Subjects
- Location of subjects
- Ireland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Posterior fornix of vagina , External cervical os Pars posterior fornicis vaginae,Posterior fornix,Posterior part of fornix of vagina,Posterior fornix of vagina,posterior fornix of vagina,External os,External os of uterus,Ostium of uterus,Ostium uteri,External cervical os,external cervical os
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Spontaneous preterm birth Spontaneous preterm birth,spontaneous preterm birth
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- No Previous Preterm birth
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Previous Preterm birth
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Pregnant women with a history of previous spontaneous preterm birth (PTB)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 29
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Women currently on antibiotic treatment.
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Linear Regression
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.25
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Source: Figure 2C
Description: Differentially abundant species for Previous Preterm birth(PTB) group
Abundance in Group 1: increased abundance in Previous Preterm birth
Revision editor(s): Joan Chuks
Signature 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-22
Source: Figure 2C
Description: Differentially abundant species for Previous Preterm birth(PTB) group
Abundance in Group 1: decreased abundance in Previous Preterm birth
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium breve | ||
| Bifidobacterium longum subsp. infantis |
Revision editor(s): Joan Chuks
