The small bowel microbiome changes significantly with age and aspects of the ageing process

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

35083314

DOI Digital object identifier for electronic documents.

10.1016/j.celrep.2021.109765

URI

https://www.sciencedirect.com/science/article/pii/S2211124721012195

Authors

Leite G, Pimentel M, Barlow GM, Mathur R

Journal

Microbial cell (Graz, Austria)

Year

2022

Keywords:

age, aging, coliforms, concomitant diseases, medication use, proteobacteria, small intestinal microbiome

Gut microbiome changes have been associated with human ageing and implicated in age-related diseases including Alzheimer's disease and Parkinson's disease. However, studies to date have used stool samples, which do not represent the entire gut. Although more challenging to access, the small intestine plays critical roles in host metabolism and immune function. In this paper (Leite et al. (2021), Cell Reports, doi: 10.1016/j.celrep.2021.109765), we demonstrate significant differences in the small intestinal microbiome in older subjects, using duodenal aspirates from 251 subjects aged 18-80 years. Differences included significantly decreased microbial diversity in older subjects, driven by increased relative abundance of phylum Proteobacteria, particularly family Enterobacteriaceae and coliform genera Escherichia and Klebsiella. Moreover, while this decreased diversity was associated with the 'ageing process' (comprising chronologic age, number of medications, and number of concomitant diseases), changes in certain taxa were found to be associated with number of medications alone (Klebsiella), number of diseases alone (Clostridium, Bilophila), or chronologic age alone (Escherichia, Lactobacillus, Enterococcus). Lastly, many taxa associated with increasing chronologic age were anaerobes. These changes may contribute to changes in human health that occur during the ageing process.

Experiment 1

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Needs review

Curated date: 2023/10/18

Curator: Davvve

Revision editor(s): Davvve

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: aging ageing,AGING BIOL,Aging, Biological,BIOL AGING,Biological Aging,Senescence,aging

Group 0 name Corresponds to the control (unexposed) group for case-control studies: group 1 ( 18 to 35 years old. young adults)

Group 1 name Corresponds to the case (exposed) group for case-control studies: group 4 ( 66 to 80 years old. elderly people)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: This is the most age advanced group from the study

Group 0 sample size Number of subjects in the control (unexposed) group: 32

Group 1 sample size Number of subjects in the case (exposed) group: 41

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances