The impact of cancer cachexia on gut microbiota composition and short-chain fatty acid metabolism in a murine model

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-1-16

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

37220908

DOI Digital object identifier for electronic documents.

10.5483/BMBRep.2023-0068

URI

Authors

Jeong SM, Jin EJ, Wei S, Bae JH, Ji Y, Jo Y, Jeong JH, Im SJ, Ryu D

Journal

BMB reports

Year

2023

This study investigates the relationship between cancer cachexia and the gut microbiota, focusing on the influence of cancer on microbial composition. Lewis lung cancer cell allografts were used to induce cachexia in mice, and body and muscle weight changes were monitored. Fecal samples were collected for targeted metabolomic analysis for short chain fatty acids and microbiome analysis. The cachexia group exhibited lower alpha diversity and distinct beta diversity in gut microbiota, compared to the control group. Differential abundance analysis revealed higher Bifidobacterium and Romboutsia, but lower Streptococcus abundance in the cachexia group. Additionally, lower proportions of acetate and butyrate were observed in the cachexia group. The study observed that the impact of cancer cachexia on gut microbiota and their generated metabolites was significant, indicating a host-to-gut microbiota axis. [BMB Reports 2023; 56(7): 404-409].

Experiment 1

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Needs review Marked as Needs review by Svetlana up on 2024-3-11

Curated date: 2023/10/08

Curator: Grace og

Revision editor(s): Grace og, Peace Sandy, Victoria

Subjects

Location of subjects: Republic of Korea

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Cachexia Wasting syndrome,Cachexia,cachexia

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: cachexia-induced mice

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: All mice that were induced with cancer cachexia (Lewis lung cancer cells).

Group 0 sample size Number of subjects in the control (unexposed) group: 3

Group 1 sample size Number of subjects in the case (exposed) group: 4

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: DESeq2

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-16

Curated date: 2023/10/08

Curator: Grace og

Revision editor(s): Grace og, Peace Sandy

Source: Fig 3

Description: Differential abundance analysis of gut microbiota by DESeq2. (B) Microbes of significantly different abundance in genus level by DESeq2 analysis between groups expressed in log2 fold change.

Abundance in Group 1: increased abundance in cachexia-induced mice

NCBI	Quality Control	Links
rumen bacterium NK4A214
Marvinbryantia
Erysipelotrichaceae
Candidatus Arthromitus
Streptococcus
Clostridium
Raoultibacter

Revision editor(s): Grace og, Peace Sandy

Signature 2

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Your review change was submitted

Reviewed Marked as Reviewed by Peace Sandy on 2024-1-16

Curated date: 2023/10/08

Curator: Grace og

Revision editor(s): Grace og, Hodan Issah, Peace Sandy

Source: Fig 3

Description: Differential abundance analysis of gut microbiota by DESeq2. (B) Microbes of significantly different abundance in genus level by DESeq2 analysis between groups expressed in log2 fold change.

Abundance in Group 1: decreased abundance in cachexia-induced mice

NCBI	Quality Control	Links
Romboutsia
Lactococcus
Turicibacter
Bifidobacterium
Ruminococcus
Colidextribacter

Revision editor(s): Grace og, Hodan Issah, Peace Sandy