Metagenomic Analysis Reveals the Heterogeneity of Conjunctival Microbiota Dysbiosis in Dry Eye Disease/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-23

Curated date: 2023/10/14

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Bulbar conjunctiva Ocular conjunctiva,Bulbar conjunctiva,bulbar conjunctiva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Biological sex Biological sex,biological sex
Group 0 name Corresponds to the control (unexposed) group for case-control studies
female patients with dry eye disease
Group 1 name Corresponds to the case (exposed) group for case-control studies
male patients with dry eye disease
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
male patients with dry eye disease
Group 0 sample size Number of subjects in the control (unexposed) group
22
Group 1 sample size Number of subjects in the case (exposed) group
25
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
90 days prior to enrollment

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Linear Regression
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-23

Curated date: 2023/10/14

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, ChiomaBlessing

Source: Supp fig 2c and 2d

Description: Sex-related differences of the conjunctival microbiota in patients with dry eye.

Abundance in Group 1: decreased abundance in male patients with dry eye disease

NCBI Quality ControlLinks
Deinococcus sp. NW-56
Pseudomonas aeruginosa

Revision editor(s): Mary Bearkland, ChiomaBlessing