Metagenomic Analysis Reveals the Heterogeneity of Conjunctival Microbiota Dysbiosis in Dry Eye Disease/Experiment 5

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Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-23

Curated date: 2023/10/15

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Victoria

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Bulbar conjunctiva Ocular conjunctiva,Bulbar conjunctiva,bulbar conjunctiva

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Dry eye syndrome dry eye,Dry Eye Syndrome,dry eye syndrome,Dry Eye Syndromes,dry eye(s),eye(s), dry,KCS,Keraconjunctivitis sicca,Keratoconjunctivitis Sicca,Keratoconjunctivitis sicca,keratoconjunctivitis sicca,Keratoconjunctivitis sicca (disorder),sicca, keratoconjunctivitis,Tear film insufficiency,tear film insufficiency,Dry eye syndrome

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Meibomian Gland Dysfunction (MGD) DED

Group 1 name Corresponds to the case (exposed) group for case-control studies

Mixed DED

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Patients with mixed type diagnosis of DED (more closely related to MGD than ATD)

Group 0 sample size Number of subjects in the control (unexposed) group

19

Group 1 sample size Number of subjects in the case (exposed) group

35

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

90 days prior to study enrollment

Lab analysis

Sequencing type

WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.15

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment

age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

unchanged