Metagenomic Analysis Reveals the Heterogeneity of Conjunctival Microbiota Dysbiosis in Dry Eye Disease/Experiment 8
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-2-23
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Bulbar conjunctiva Ocular conjunctiva,Bulbar conjunctiva,bulbar conjunctiva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Dry eye syndrome dry eye,Dry Eye Syndrome,dry eye syndrome,Dry Eye Syndromes,dry eye(s),eye(s), dry,KCS,Keraconjunctivitis sicca,Keratoconjunctivitis Sicca,Keratoconjunctivitis sicca,keratoconjunctivitis sicca,Keratoconjunctivitis sicca (disorder),sicca, keratoconjunctivitis,Tear film insufficiency,tear film insufficiency,Dry eye syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Dry Eye -nonMGD
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Dry Eye- MGD
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with Dry eye disease characterized as meibomian gland disorder
- Group 0 sample size Number of subjects in the control (unexposed) group
- 49
- Group 1 sample size Number of subjects in the case (exposed) group
- 19
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 90 days prior to study enrollment
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- MaAsLin2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.1
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-2-23
Source: Figure 4c
Description: Model coefficients of top-ranked species associated with either MGD or non-MGD dry eye (p < 0.1, coefficient >0.2)
Abundance in Group 1: increased abundance in Dry Eye- MGD
| NCBI | Quality Control | Links |
|---|---|---|
| Acinetobacter sp. WCHA45 | ||
| Deinococcus sp. NW-56 | ||
| Staphylococcus aureus | ||
| Staphylococcus capitis | ||
| Staphylococcus cohnii |
Revision editor(s): Mary Bearkland
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-2-23
Source: Figure 4c
Description: Model coefficients of top-ranked species associated with either MGD or non-MGD dry eye (p < 0.1, coefficient >0.2)
Abundance in Group 1: decreased abundance in Dry Eye- MGD
| NCBI | Quality Control | Links |
|---|---|---|
| Collinsella aerofaciens |
Revision editor(s): Mary Bearkland
