COVID-19 mRNA vaccine-mediated antibodies in human breast milk and their association with breast milk microbiota composition/Experiment 4
From BugSigDB
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-15
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Breast , Milk Mamma,Mammary part of chest,Mammary region,Breast,breast,Mammary gland milk,Milk,milk
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to vaccine Response to vaccine,response to vaccine
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Mothers at pre-vaccination timepoint (baseline)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mothers at one week post-first dose
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Breastfeeding mothers at one week after receiving the first dose of BNT162b2 vaccine.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 44
- Group 1 sample size Number of subjects in the case (exposed) group
- 44
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 1.5
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
Signature 1
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-15
Source: supplementary table 3
Description: differential abundance of mothers one week after first dose of BNT162b2 when compared to baseline.
Abundance in Group 1: increased abundance in Mothers at one week post-first dose
Revision editor(s): Folakunmi, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-15
Source: Supplementary Table 3
Description: Differential abundance of mothers one week after first dose of BNT162b2 when compared to baseline.
Abundance in Group 1: decreased abundance in Mothers at one week post-first dose
Revision editor(s): ChiomaBlessing
