Yearly variation coupled with social interactions shape the skin microbiome in free-ranging rhesus macaques/Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Macaca mulatta
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Axilla skin Axillary skin,Skin of axilla,Axilla skin,axilla skin
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Microbiome measurement Microbiome measurement,microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- 2013 sampling period
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- 2015 sampling period
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The 2015 sampling period occurred from October 2015 through December 2015
- Group 0 sample size Number of subjects in the control (unexposed) group
- 42
- Group 1 sample size Number of subjects in the case (exposed) group
- 33
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- Mann-Whitney (Wilcoxon)
- Welch's T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- Confounders controlled for: "sampling period" is not in the list (abnormal glucose tolerance, acetaldehyde, acute graft vs. host disease, acute lymphoblastic leukemia, acute myeloid leukemia, adenoma, age, AIDS, alcohol consumption measurement, alcohol drinking, ...) of allowed values.sampling period
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig 6, Text
Description: The volcano plot (center) depicts the differences between the 2013 and 2015 sampling periods for each detected ASV (red and black points). Taxa depicted in red are the most highly significant (P < 0.015). Overabundant ASVs from 2013 are located on the negative (left) side of the volcano plot, while overabundant ASVs from 2015 are located on the positive (right) side. The violin plots surrounding the volcano plot depict the CLR values of the most significant taxa from the volcano plot; left: overabundant taxa in 2013; right: overabundant taxa in 2015
Abundance in Group 1: increased abundance in 2015 sampling period
Revision editor(s): Fiddyhamma
Signature 2
Source: Fig 6, Text
Description: The volcano plot (center) depicts the differences between the 2013 and 2015 sampling periods for each detected ASV (red and black points). Taxa depicted in red are the most highly significant (P < 0.015). Overabundant ASVs from 2013 are located on the negative (left) side of the volcano plot, while overabundant ASVs from 2015 are located on the positive (right) side. The violin plots surrounding the volcano plot depict the CLR values of the most significant taxa from the volcano plot; left: overabundant taxa in 2013; right: overabundant taxa in 2015
Abundance in Group 1: decreased abundance in 2015 sampling period
| NCBI | Quality Control | Links |
|---|---|---|
| Cecembia | ||
| Nocardioides aestuarii | ||
| Paracoccus sp. (in: a-proteobacteria) | ||
| Rickettsiella sp. | ||
| Thauera aromatica |
Revision editor(s): Fiddyhamma
