Taxonomic Characterization and Short-Chain Fatty Acids Production of the Obese Microbiota/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-15
Subjects
- Location of subjects
- Spain
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal weight volunteers (N)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Obese Volunteers (O)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Obese adult volunteers with body mass index (BMI) > 30 kg/m^2
- Group 0 sample size Number of subjects in the control (unexposed) group
- 13
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Volunteers who were on antibiotics six (6) months preceding the sample collection were excluded from the study
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-15
Source: Figure 5
Description: The significant differences in gut microbiota composition in normal weight (N) and obese (O) subjects.
Abundance in Group 1: increased abundance in Obese Volunteers (O)
| NCBI | Quality Control | Links |
|---|---|---|
| Abiotrophia | ||
| Aerococcaceae | ||
| Catenibacterium | ||
| Collinsella | ||
| Coprobacillus | ||
| Negativicutes | ||
| Selenomonadales | ||
| Slackia | ||
| Solobacterium |
Revision editor(s): Aleru002, Peace Sandy
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-1-15
Source: Figure 5
Description: The significant differences in gut microbiota composition in normal weight (N) and obese (O) subjects.
Abundance in Group 1: decreased abundance in Obese Volunteers (O)
Revision editor(s): Aleru002, Peace Sandy
