Geography, niches, and transportation influence bovine respiratory microbiome and health/Experiment 7

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-1-28

Curated date: 2023/11/14

Curator: Folakunmi

Revision editor(s): Folakunmi, ChiomaBlessing

Subjects

Location of subjects
Canada
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Bos taurus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Nasopharynx Nasenrachenraum,Epipharynx,Nasal part of pharynx,Pars nasalis pharyngis,Rhinopharynx,Nasopharynx,nasopharynx
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Transport Transport,transport
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-transportation (control)
Group 1 name Corresponds to the case (exposed) group for case-control studies
before loading for long distance transportation
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
calves before loading into truck for long distance transportation. LefSe was used to identify the longitudinal changes of microbiota from the studies of Cui et al. (2021) and Malmuthuge et al. (2021), following transportation.
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
none

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-1-28

Curated date: 2023/11/14

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: supplementary figure S4

Description: calves before loading for long distance transportation. LefSe was used to identify the longitudinal changes of microbiota from the studies of Cui et al. (2021) and Malmuthuge et al. (2021), following transportation.

Abundance in Group 1: increased abundance in before loading for long distance transportation

NCBI Quality ControlLinks
Simonsiella muelleri
Methylobacterium radiotolerans
Pseudomonas fluorescens
Rhizorhabdus wittichii
Methylorubrum extorquens

Revision editor(s): Folakunmi