Metagenomic analysis of microbiological changes on the ocular surface of diabetic children and adolescents with a dry eye/Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Conjunctival sac Conjunctiva serous sac,Subbrillar sac,Conjunctival sac,conjunctival sac
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Dry eye syndrome dry eye,Dry Eye Syndrome,dry eye syndrome,Dry Eye Syndromes,dry eye(s),eye(s), dry,KCS,Keraconjunctivitis sicca,Keratoconjunctivitis Sicca,Keratoconjunctivitis sicca,keratoconjunctivitis sicca,Keratoconjunctivitis sicca (disorder),sicca, keratoconjunctivitis,Tear film insufficiency,tear film insufficiency,Dry eye syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal healthy (NDM) children
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Diabetic children with Dry Eye Disease (DM-DE)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Children aged 8-16 with Diabetes and Dry Eye Disease
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- Matched on Factors on which subjects have been matched on in a case-control study
- age, body mass index, sex
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Signature 1
Source: Figure 3g and Results within text (Page 7, Last 6 lines, Under subheading: Composition and difference analysis of the ocular microbiome)
Description: Fig 3g: Species composition with significant difference at the species level between the NDM and DM-DE groups (top 20)
Abundance in Group 1: decreased abundance in Diabetic children with Dry Eye Disease (DM-DE)
Revision editor(s): Mary Bearkland, ChiomaBlessing
Signature 2
Source: Figure 3g and Results within text (Page 7, Last 6 lines, Under subheading: Composition and difference analysis of the ocular microbiome)
Description: Figure 3g Species composition with significant difference at the species level between the NDM and DM-DE groups (top 20)
Abundance in Group 1: increased abundance in Diabetic children with Dry Eye Disease (DM-DE)
| NCBI | Quality Control | Links |
|---|---|---|
| Cordyceps militaris | ||
| Enterobacter mori | ||
| Leuconostoc sp. DORA_2 | ||
| Salmonella enterica | ||
| Vibrio vulnificus |
Revision editor(s): Mary Bearkland, ChiomaBlessing
