Resilience of the respiratory microbiome in controlled adult RSV challenge study

From BugSigDB
Needs review
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Cuthbertson L, James P, Habibi MS, Thwaites RS, Paras A, Chiu C, Openshaw PJM, Cookson WOC, Moffatt MF
The European respiratory journal
This study of healthy adults revealed no major changes in the bacterial community of the respiratory tracts following RSV inoculation, suggesting that the adult respiratory microbial community is resilient to viral perturbations

Experiment 1

Needs review

Curated date: 2024/03/10

Curator: Aleru Divine

Revision editor(s): Aleru Divine


Location of subjects
United Kingdom
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Throat , Nasal cavity Gula,Throat,throat,Cavitas nasalis,Cavitas nasi,Cavity of nose,Cavity of olfactory apparatus,Cavum nasi,Nasal canal,Nasal conduit space,Nasal fossa,Nasal pit,Olfactory cavity,Olfactory chamber,Olfactory chamber cavity,Olfactory pit,Nasal cavity,nasal cavity
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Respiratory Syncytial Virus Infection Human respiratory syncytial virus infection,infections, Human respiratory syncytial virus,respiratory syncytial virus infection,respiratory syncytial virus infectious disease,Respiratory Syncytial Virus Infection,respiratory Syncytial Virus Infection
Group 1 name Corresponds to the case (exposed) group for case-control studies
Healthy non-smoking adults
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Healthy non-smoking adults between 18 and 50 years of age.
Group 1 sample size Number of subjects in the case (exposed) group

Lab analysis

Sequencing type
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
Simpson Estimator of species richness and species evenness: more weight on species evenness
Richness Number of species