Microbiome features associated with performance measures in athletic and non-athletic individuals: A case-control study
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Poland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Health study participation health questionnaire participation,health survey participation,Health study participation,health study participation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-athletes
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Strength athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in strength athletics at timepoint B0 (in the morning before the Bruce Treadmill Test)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 19
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Individuals who were on antibiotics six (6) months before the start of the study.
Lab analysis
- Sequencing type
- PCR
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- diet
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint B0 (in the morning before the Bruce Treadmill Test)
Abundance in Group 1: increased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Catenibacterium sp. AM22-15 | ||
| Clostridium phoceensis |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endurance athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in endurance athletics (in the morning before the Bruce Treadmill Test)
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at time point B0(in the morning before the Bruce Treadmill Test)
Abundance in Group 1: increased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. AF19-10LB | ||
| Dorea longicatena |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 3
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Strength athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in strength athletics at timepoint W1 (the same day after the repeated lower body 30s-all-out wingate's test)
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W1 (the same day after the repeated lower body 30s-all-out wingate's test)
Abundance in Group 1: increased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Coprococcus catus | ||
| Rothia mucilaginosa | ||
| Ruminococcus sp. AF41-9 |
Revision editor(s): Aleru Divine, Folakunmi
Signature 2
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W1 (the same day after the repeated lower body 30s-all-out wingate's test)
Abundance in Group 1: decreased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides faecis |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 4
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endurance athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in endurance athletics at time point W1 (the same day after the repeated lower body 30s-all-out wingate's test)
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at time point W1 (the same day after the repeated lower body 30s-all-out wingate's test)
Abundance in Group 1: increased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Fusicatenibacter saccharivorans |
Revision editor(s): Aleru Divine, Folakunmi
Signature 2
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W1 (the same day after the repeated lower body 30s-all-out wingate's test)
Abundance in Group 1: decreased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides faecis |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 5
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Strength athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in strength athletics at timepoint W2 (morning fasting after the WT on an empty stomach)
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W2 (morning fasting after the WT on an empty stomach)
Abundance in Group 1: decreased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Phocaeicola massiliensis |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 6
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endurance athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in endurance athletics at timepoint W2 (morning fasting after the WT on an empty stomach)
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W2 (morning fasting after the WT on an empty stomach)
Abundance in Group 1: increased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. AF19-10LB |
Revision editor(s): Aleru Divine, Folakunmi
Signature 2
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint W2 (morning fasting after the WT on an empty stomach)
Abundance in Group 1: decreased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Phocaeicola massiliensis |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 7
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Strength athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in strength athletics at timepoint B1 (the same day after the Bruce Treadmill Test)
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at timepoint B1 (the same day after the Bruce Treadmill Test)
Abundance in Group 1: increased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridiales bacterium |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 8
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endurance athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in endurance athletics at timepoint B1 (the same day after the Bruce Treadmill Test)
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at timepoint B1 (the same day after the Bruce Treadmill Test)
Abundance in Group 1: increased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. AF19-10LB | ||
| Lacticaseibacillus paracasei |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 9
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Strength athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in strength athletics at timepoint B2 (morning after the Bruce Treadmill Test on an empty stomach)
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint B2 (morning after the Bruce Treadmill Test on an empty stomach)
Abundance in Group 1: increased abundance in Strength athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. MSK.20.85 |
Revision editor(s): Aleru Divine, Folakunmi
Experiment 10
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endurance athletes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Athletes engaged in endurance athletics at timepoint B2 (morning after the Bruce Treadmill Test on an empty stomach)
- Group 1 sample size Number of subjects in the case (exposed) group
- 13
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Source: Table 2
Description: Species identified as specific per group after being identified as significantly more abundant in the group of interest in comparison to the other two groups by LEfSe at Timepoint B2 (morning after the Bruce Treadmill Test)
Abundance in Group 1: increased abundance in Endurance athletes
| NCBI | Quality Control | Links |
|---|---|---|
| Blautia sp. AF19-10LB |
Revision editor(s): Aleru Divine, Folakunmi
