Efficacy of fecal microbiota transplantation in 2 children with recurrent Clostridium difficile infection and its impact on their growth and gut microbiome

Study information

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study design

case-control

Citation

PMID PubMed identifier for scientific articles.

25023578

DOI Digital object identifier for electronic documents.

https://doi.org/10.1097/MPG.0000000000000495

URI

Authors

Walia R, Garg S, Song Y, Girotra M, Cuffari C, Fricke WF, Dutta SK

Journal

Journal of pediatric gastroenterology and nutrition

Year

2014

Fecal microbiota transplantation (FMT) is recognized as an alternative therapeutic modality for recurrent Clostridium difficile infection (RCDI); however, data on its efficacy in children are lacking, including its effect on their growth and fecal microbiota. We report on 2 young children (<3 years old) who failed available therapeutics for RCDI, but responded remarkably well to FMT. Besides resolution of clinical features of C difficile infection (CDI), FMT administration led to marked improvement in their growth, along with increased microbiota diversity, especially proportion of Bacteroides. Our 2 cases illustrate the efficacy of FMT in children with RCDI and its positive effect on their growth and gut microbiota.

Experiment 1

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Curated date: 2024/03/05

Curator: Victoria

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Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Clostridium difficile infection clostridium difficile disease,Clostridium difficile infection,clostridium difficile infection

Group 1 name Corresponds to the case (exposed) group for case-control studies: Children with recurrent Clostridium difficile Infection

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Efficacy of Fecal Microbiota Transplantation on Children with Recurrent Clostridium difficile Infection.

Group 1 sample size Number of subjects in the case (exposed) group: 2

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: age, cesarean section, premature birth, antibiotic exposure

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Signature 1

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Source: Figure 2b

Description: Histograms showing relative abundances of microbiota members at the taxonomic phylum level.

Abundance in Group 1: increased abundance in Children with recurrent Clostridium difficile Infection

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Bacteroidota

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Signature 2

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Source: Figure 2b

Description: Histograms showing relative abundances of microbiota members at the taxonomic phylum level.

Abundance in Group 1: decreased abundance in Children with recurrent Clostridium difficile Infection

NCBI	Quality Control	Links
Bacillota
Pseudomonadota
Verrucomicrobiota
Actinomycetota

Revision editor(s): Victoria