Patients with Acne Vulgaris Have a Distinct Gut Microbiota in Comparison with Healthy Controls

Study information

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-3-14

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

29756631

DOI Digital object identifier for electronic documents.

10.2340/00015555-2968

URI

Authors

Deng Y, Wang H, Zhou J, Mou Y, Wang G, Xiong X

Journal

Acta dermato-venereologica

Year

2018

Acne vulgaris has been postulated to have a gastrointestinal mechanism; however, little is known about gut microbiota dysfunction in this condition. The aim of this cross-sectional study was to investigate whether the gut microbiota is altered in acne. Faecal bacterial diversity was analysed in 43 patients with acne and 43 controls, using hypervariable tag sequencing of the V3-V4 region of the 16S rDNA gene. Distinct differences were found in microbial diversity between patients with acne and controls (Shannon diversity index (p = 0.009) and Simpson diversity index (p = 0.01)). At the phylum level, the abundance of Firmicutes was lower in the patient group, but that of Bacteroidiain was higher. The most significantly depleted taxa in acne were Clostridia, Clostridiales, Lachnospiraceae and Ruminococcaceae genera, which are potentially beneficial. In conclusion, patients with acne vulgaris have gut microbial dysbiosis; further study is needed to understand its role in the pathogenesis of acne.

Experiment 1

Edit History Delete

Mark reviewed

Your review change was submittedDuplicate this Experiment Add a new Signature

Needs review

Curated date: 2024/03/05

Curator: Scholastica

Revision editor(s): WikiWorks, ChiomaBlessing, Scholastica

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Acne acne,acne (disease),acne varioliformis,Acne Vulgaris,acne vulgaris,acne, ADULT,frontalis acne,Acne

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy control group

Group 1 name Corresponds to the case (exposed) group for case-control studies: Acne vulgaris group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with mild, moderate, severe and very severe acne

Group 0 sample size Number of subjects in the control (unexposed) group: 43

Group 1 sample size Number of subjects in the case (exposed) group: 43

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness: decreased

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-3-14

Curated date: 2024/03/06

Curator: Scholastica

Revision editor(s): Scholastica, ChiomaBlessing, WikiWorks

Source: Figure 3A

Description: Bacterial taxa significantly enriched in acne samples (AS) compared to healthy control samples (CS)

Abundance in Group 1: increased abundance in Acne vulgaris group

NCBI	Quality Control	Links
Bacteroidales
Bacteroidia
Bacteroidota

Revision editor(s): Scholastica, ChiomaBlessing, WikiWorks

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-3-14

Curated date: 2024/03/06

Curator: Scholastica

Revision editor(s): Scholastica, ChiomaBlessing, WikiWorks

Source: Figure 3A

Description: Bacterial taxa significantly abundant in acne samples (AS) compared to healthy control samples (CS)

Abundance in Group 1: decreased abundance in Acne vulgaris group

NCBI	Quality Control	Links
Aerococcaceae
Aerococcus
Alkaliphilus
Bacillaceae
Bacillales
Bacillus
Bilophila
Blautia
Butyricicoccus
Carnobacteriaceae
Carnobacterium
Clostridia
Clostridiaceae
Deltaproteobacteria
Desulfovibrionaceae
Desulfovibrionales
Enhydrobacter
Fusicatenibacter
Gemmiger
Granulicatella
Lachnospiraceae
Lachnospiraceae incertae sedis
Lactococcus
Lysinibacillus
Oceanobacillus
Oxalobacter
Paenibacillaceae
Paenibacillus
Paraprevotella
Caryophanaceae
Ruminococcus
Bacillota
Eubacteriales
Oscillospiraceae

Revision editor(s): Scholastica, ChiomaBlessing, WikiWorks