Oral microbiome and preterm birth

From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-12
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Vidmar Šimic M, Maver A, Zimani AN, Hočevar K, Peterlin B, Kovanda A, Premru-Sršen T
Journal
Frontiers in medicine
Year
2023
Keywords:
16S rDNA, 16S rRNA gene, microbiome, oral microbiome, pregnancy, preterm delivery
BACKGROUND: The etiology of preterm birth (PTB) is heterogeneous and not yet well known. Maternal periodontal disease has been investigated for decades and is a known risk factor for adverse pregnancy outcomes. However, no particular bacterial species or higher taxonomic order has been found as causative of PTB, leading to studies of the whole oral microbiome. In order to determine if and how the composition of the oral microbiome is associated with PTB, we performed a large case-control study including women with term (TB) and PTB. METHODS: We compared oral microbiomes in PTB to TB, to examine differences in the microbial richness, diversity, and differential abundance of specific taxa. We obtained oral swab samples from 152 Caucasian pregnant women who were classified as either PTB (≤36 6/7 weeks, n = 61) or TB (≥38 0/7 weeks, n = 91) in exclusion of any other major medical or obstetric conditions. The oral microbiomes of these women were characterized by 16S ribosomal RNA (rRNA) gene sequencing of the V3-V4 region on the MiSeq platform. RESULTS: The dominant microorganisms at the phylum level in all pregnant women regardless of birth week outcomes as belonging to Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, and Actinobacteria. The phyla Firmicutes and Bacteroidetes were relatively more abundant in women with a PTB than in women with a TB, while Proteobacteria was less prevalent in women with a PTB. At the genus level, Veillonella, Prevotella, and Capnocytophaga were enriched in the PTB, and while many of the members of these genera could not be resolved to the species level, Veillonella massillensis was shown to be increased in the PTB group. CONCLUSION: We identified the genera Veillonella, Prevotella, and Capnocytophaga in the maternal oral microbiome as being associated with PTB independently of clinically apparent infection, uterine anomalies, and other pregnancy complications, including placenta previa, and placental abruption. The clarification of the role of those taxa in the etiology of PTB merits further research.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-6-12

Curated date: 2024/04/06

Curator: Ayibatari

Revision editor(s): Ayibatari, Victoria, Scholastica

Subjects

Location of subjects
Slovenia
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Oral cavity Bucca,Buccal cavity,Cavity of mouth,Oral cavity,oral cavity
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Premature birth Birth, Premature,Birth, Preterm,Births, Premature,Births, Preterm,Premature Births,Preterm Birth,Preterm Births,Premature birth,premature birth
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Term birth (TB)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Preterm birth (PTB)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Spontaneous onset of preterm labor (≤36 6/7 weeks) due to various causes, including spontaneous contractions, preterm premature rupture of membranes (PPROM), intrauterine infection, cervical insufficiency, and others
Group 0 sample size Number of subjects in the control (unexposed) group
91
Group 1 sample size Number of subjects in the case (exposed) group
61
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
Week before inclusion

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-6-12

Curated date: 2024/04/06

Curator: Ayibatari

Revision editor(s): Ayibatari, Scholastica

Source: Figure 4

Description: Differential relative abundance of oral bacteria in term vs. preterm delivery groups

Abundance in Group 1: increased abundance in Preterm birth (PTB)

NCBI Quality ControlLinks
Capnocytophaga
Veillonella
Veillonella massiliensis
Prevotella_7Prevotella_7

Revision editor(s): Ayibatari, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-6-12

Curated date: 2024/06/11

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 4

Description: Differential relative abundance of oral bacteria in term vs. preterm delivery groups

Abundance in Group 1: decreased abundance in Preterm birth (PTB)

NCBI Quality ControlLinks
Corynebacterium
Actinomyces
Lachnoanaerobaculum
Actinomyces sp. oral taxon 848 str. F0332
Campylobacter
Alloprevotella
Porphyromonas
Catonella
Leptotrichia
Veillonella
Neisseria
Streptococcus
Rothia
Rothia dentocariosa
Gemella
Haemophilus
Streptococcus australis
Streptococcus infantis
Streptococcus mitis
Streptococcus oralis
Streptococcus sanguinis
Haemophilus influenzae
Haemophilus parainfluenzae
Streptococcus cristatus
Gemella sanguinis
Leptotrichia wadei
Haemophilus sputorum
Leptotrichia trevisanii
Lachnoanaerobaculum saburreum
Campylobacter gracilis
Streptococcus pneumoniae
Neisseria oralis
Streptococcus phageStreptococcus phage
Neisseria subflava
Neisseria mucosa
Rothia mucilaginosa
Haemophilus haemolyticus

Revision editor(s): Scholastica