Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation/Experiment 1
Subjects
- Location of subjects
- Brazil
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Supragingival dental plaque Supragingival plaque,Supragingival dental plaque,supragingival dental plaque
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to allogeneic hematopoietic stem cell transplant Response to allogeneic hematopoietic stem cell transplant,response to allogeneic hematopoietic stem cell transplant
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Preconditioning (Patients Before Allogeneic Hematopoietic Stem Cell Transplantation)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Engraftment (Patients After Allogeneic Hematopoietic Stem Cell Transplantation)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients at the final phase of Allogeneic Hematopoietic Stem Cell transplant, when the new transplanted stem cells start to grow and produce new blood cells.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 27
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- No
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- centered log-ratio
- Statistical test
- ANCOM
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.7
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
Signature 1
Source: Figure 1C
Description: Significant genera relative abundance in the engraftment (After transplantation) group compared to the preconditioning (Before transplantation) group according to ANCOM test (W > 0.7).
Abundance in Group 1: increased abundance in Engraftment (Patients After Allogeneic Hematopoietic Stem Cell Transplantation)
| NCBI | Quality Control | Links |
|---|---|---|
| Enterococcus | ||
| Lactobacillus | ||
| Leuconostoc | ||
| Mycoplasma | ||
| Staphylococcus |
Revision editor(s): Joan Chuks, ChiomaBlessing
Signature 2
Source: Figure 1C
Description: Significant genera relative abundance in the engraftment (After transplantation) group compared to the preconditioning (Before transplantation) group according to ANCOM test (W > 0.7).
Abundance in Group 1: decreased abundance in Engraftment (Patients After Allogeneic Hematopoietic Stem Cell Transplantation)
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomyces | ||
| Gemella | ||
| Lachnoanaerobaculum | ||
| Leptotrichia | ||
| Streptococcus |
Revision editor(s): Joan Chuks, ChiomaBlessing
