Dental Biofilm Microbiota Dysbiosis Is Associated With the Risk of Acute Graft-Versus-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation/Experiment 2

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Reviewed Marked as Reviewed by ChiomaBlessing on 2024-3-14

Curated date: 2024/03/14

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing, Victoria

Subjects

Location of subjects

Brazil

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Supragingival dental plaque Supragingival plaque,Supragingival dental plaque,supragingival dental plaque

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Response to allogeneic hematopoietic stem cell transplant Response to allogeneic hematopoietic stem cell transplant,response to allogeneic hematopoietic stem cell transplant

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Preconditioning (Patients Before Allogeneic Hematopoietic Stem Cell Transplantation)

Group 1 name Corresponds to the case (exposed) group for case-control studies

Aplasia group

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Patients after the conditioning regimen, when the patient's bone marrow is intentionally depleted of cells, necessary for the success of the transplant

Group 0 sample size Number of subjects in the control (unexposed) group

30

Group 1 sample size Number of subjects in the case (exposed) group

30

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

No

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

centered log-ratio

Statistical test

ANCOM

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.7

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

decreased

Simpson Estimator of species richness and species evenness: more weight on species evenness

decreased

Richness Number of species

decreased