Maternal influence on the fetal microbiome in a population-based study of the first-pass meconium
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Finland
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Meconium Meconium
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- cesarean section caesarean section,cesarean section
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- vaginal delivery
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- C-section
- Group 0 sample size Number of subjects in the control (unexposed) group
- 172
- Group 1 sample size Number of subjects in the case (exposed) group
- 40
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- newborns administerated antibiotics during delivery: Cefuroxime (n= 31), penicillin (n= 28), piperacillin-tazobactam (n=2)
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Table 3
Description: Effects of delivery mode on the microbiome of the first stool.
Abundance in Group 1: decreased abundance in C-section
NCBI | Quality Control | Links |
---|---|---|
Staphylococcus |
Revision editor(s): WikiWorks
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- no
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Antimicrobials use during delivery yes
- Group 0 sample size Number of subjects in the control (unexposed) group
- 151
- Group 1 sample size Number of subjects in the case (exposed) group
- 61
Lab analysis
Statistical Analysis
Signature 1
Source: Table 3
Description: Effect of antimicrobial use on the microbioome of the first stool
Abundance in Group 1: increased abundance in Antimicrobials use during delivery yes
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium |
Revision editor(s): WikiWorks
Experiment 3
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Maternal consumption of probiotics yes
Lab analysis
Statistical Analysis
Signature 1
Source: Table 3
Description: Effect of probiotics during pregnancy on the microbioome of the first stool
Abundance in Group 1: increased abundance in Maternal consumption of probiotics yes
NCBI | Quality Control | Links |
---|---|---|
Lactobacillus |
Revision editor(s): WikiWorks
Experiment 4
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Furry pets at home yes
- Group 0 sample size Number of subjects in the control (unexposed) group
- 108
- Group 1 sample size Number of subjects in the case (exposed) group
- 104
Lab analysis
Statistical Analysis
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- delivery procedure, antimicrobial agent during labor, probiotics during pregnancy
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 1
Source: Table 4
Description: mulivariate analysis adjusting for furry pets at home
Abundance in Group 1: increased abundance in Furry pets at home yes
NCBI | Quality Control | Links |
---|---|---|
Bacteroidota | ||
Faecalibacterium |
Revision editor(s): WikiWorks
Signature 2
Source: Table 4
Description: mulivariate analysis adjusting for furry pets at home
Abundance in Group 1: decreased abundance in Furry pets at home yes
NCBI | Quality Control | Links |
---|---|---|
Staphylococcus | ||
Bacillota |
Revision editor(s): WikiWorks