Maternal influence on the fetal microbiome in a population-based study of the first-pass meconium

From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Tapiainen T, Paalanne N, Tejesvi MV, Koivusaari P, Korpela K, Pokka T, Salo J, Kaukola T, Pirttilä AM, Uhari M, Renko M
Journal
Pediatric research
Year
2018
BACKGROUND: Meconium is formed before birth and may reflect the microbiome of the fetus. To test our hypothesis, we investigated whether maternal factors during pregnancy, such as biodiversity of the living environment, influence the microbiome of the first stool more than immediate perinatal factors. METHODS: We recruited 218 consecutive newborn infants from one hospital. Regions of the bacterial 16S rRNA gene were sequenced to characterize the microbiomes of the first-pass meconium samples (N=212). We used a multivariate model to determine both the prenatal and perinatal factors affecting the microbiome. RESULTS: The number of operational taxonomic units ranged from 0 to 448 per newborn. The most abundant phyla were Firmicutes, with a relative abundance of 44%, Proteobacteria, 28%, and Bacteroidetes, 15%. By a multivariate analysis, the biodiversity of the home environment increased the diversity of microbiomes, whereas perinatal factors, such as the delivery mode or exposure to antimicrobials during labor did not have an effect. CONCLUSION: The microbiome of the first-pass meconium was not altered by immediate perinatal factors, but was affected by maternal factors during pregnancy, implying the in utero transfer of microbes and the development of the gut microbiota niche in fetal life.

Experiment 1


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): Shaimaa, WikiWorks, ChiomaBlessing

Subjects

Location of subjects
Finland
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Meconium Meconium,meconium
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cesarean section caesarean section,Cesarean section,cesarean section
Group 0 name Corresponds to the control (unexposed) group for case-control studies
vaginal delivery
Group 1 name Corresponds to the case (exposed) group for case-control studies
C-section
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Delivery via cesarean section
Group 0 sample size Number of subjects in the control (unexposed) group
172
Group 1 sample size Number of subjects in the case (exposed) group
40
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4-V5
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 3

Description: Effect of delivery mode on the microbiome in the CS group compared to the vaginal delivery group

Abundance in Group 1: decreased abundance in C-section

NCBI Quality ControlLinks
Staphylococcus

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 2


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Antimicrobials use during delivery (no)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Antimicrobials use during delivery (yes)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mothers who used antimicrobials during delivery (Cefuroxime (n= 31), penicillin (n= 28), piperacillin-tazobactam (n=2))
Group 0 sample size Number of subjects in the control (unexposed) group
151
Group 1 sample size Number of subjects in the case (exposed) group
61
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None.

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 3

Description: Effect of antimicrobial use on the microbiome of the first stool in the group who received antimicrobials (YES) compared to those did not (NO)

Abundance in Group 1: increased abundance in Antimicrobials use during delivery (yes)

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 3


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Maternal consumption of probiotics (NO)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Maternal consumption of probiotics (YES)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mothers who consumed probiotics (lactobacilli) during pregnancy

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 3

Description: Effect of probiotics on the microbiome in the group who consumed lactobacilli (YES) compared to the group who did not (NO)

Abundance in Group 1: increased abundance in Maternal consumption of probiotics (YES)

NCBI Quality ControlLinks
Lactobacillus

Revision editor(s): WikiWorks, ChiomaBlessing

Experiment 4


Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Furry pets at home (NO)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Furry pets at home (YES)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mothers who have furry pets at home during pregnancy
Group 0 sample size Number of subjects in the control (unexposed) group
108
Group 1 sample size Number of subjects in the case (exposed) group
104

Lab analysis

Statistical Analysis

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
antimicrobial agent during labor, delivery procedure, probiotics during pregnancy

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 4

Description: Effect of furry pets at home on the microbiome in the group who have furry pets at home (YES) compared to the group who don't have (NO)

Abundance in Group 1: increased abundance in Furry pets at home (YES)

NCBI Quality ControlLinks
Bacteroidota
Faecalibacterium

Revision editor(s): WikiWorks, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Shaimaa Elsafoury

Revision editor(s): WikiWorks, ChiomaBlessing

Source: Table 4

Description: Effect of furry pets at home on the microbiome in the group who have furry pets at home (YES) compared to the group who don't have (NO)

Abundance in Group 1: decreased abundance in Furry pets at home (YES)

NCBI Quality ControlLinks
Bacillota
Staphylococcus

Revision editor(s): WikiWorks, ChiomaBlessing