Differential Analysis of Gut Microbiota Correlated With Oxidative Stress in Sows With High or Low Litter Performance During Lactation
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Wang H, Ji Y, Yin C, Deng M, Tang T, Deng B, Ren W, Deng J, Yin Y, Tan C
Journal
Frontiers in microbiology
Year
2018
Keywords:
gut microbiota, lactation, litter performance, oxidative stress, sows
It has been suggested that gut microbiota play a critical role in maternal metabolic oxidative stress responses and offspring growth. However, whether the gut microbiota and oxidative stress status of the sows affect the litter performance during lactation is unclear. A total of 66 Yorkshire sows were identified as high (H) or low (L) litter performance sows based on litter weight at day 21 of lactation. Ten sows per group with similar parity, backfat thickness, and litter weight after cross-foster from the H or L group were collected randomly to analyze the oxidative stress and gut microbiota during lactation. The result showed that the serum total antioxidant capacity was higher in the H group, while 8-hydroxy-deoxyguanosine and thiobarbituric acid reactive substances were lower in the H group at farrowing. Four distinct clusters of bacteria were related to litter performance and reproductive periods of sows. Twelve differentially abundant taxa during gestation and 13 taxa during lactation were identified as potential biomarkers between the H group and the L group. Moreover, the litter performance and the antioxidant capacity of sows were positively correlated with Bacteroides_f__Bacteroidaceae but negatively with Phascolarctobacterium and Streptococcus. In conclusion, this study found that gut microbiota and oxidative stress were significantly correlated with the litter performance of sows during lactation.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Sus scrofa domesticus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High litter performance sows at G109
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Low litter performance sows at G109
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Low litter performance sows group at day 109 of gestation
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4B
Description: Differences in fecal microbiota in day 109 of gestation of high litter performance sows compared to day 109 of gestation of low litter performance sows
Abundance in Group 1: increased abundance in Low litter performance sows at G109
Revision editor(s): Scholastica
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4B
Description: Differences in fecal microbiota in day 109 of gestation of high litter performance sows compared to day 109 of gestation of low litter performance sows
Abundance in Group 1: decreased abundance in Low litter performance sows at G109
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidaceae | ||
| Bacteroides |
Revision editor(s): Scholastica
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- High litter performance sows at L21
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Low litter performance sows at L21
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Low litter performance sows group at day 21 of lactation (L21)
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4E
Description: Differences in fecal microbiota in day 21 of lactation of high litter performance sows compared to day 21 of lactation of low litter performance sows
Abundance in Group 1: increased abundance in Low litter performance sows at L21
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridia | ||
| Eubacteriales |
Revision editor(s): Scholastica
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4E
Description: Differences in fecal microbiota in day 21 of lactation of high litter performance sows compared to day 21 of lactation of low litter performance sows
Abundance in Group 1: decreased abundance in Low litter performance sows at L21
| NCBI | Quality Control | Links |
|---|---|---|
| Coprococcus | ||
| Mollicutes | ||
| Mycoplasmatota | ||
| Parabacteroides | ||
| Porphyromonadaceae | ||
| unclassified Mollicutes | ||
| o_RF39o_RF39 | ||
| f_norank_o_RF39f_norank_o_RF39 | ||
| g_norank_o_RF39g_norank_o_RF39 |
Revision editor(s): Scholastica
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 109 of gestation (G109) of H group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 21 of lactation (L21) of H group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Day 21 of lactation (L21) of high litter performance sows group
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4H
Description: Differences in fecal microbiota in day 109 of gestation (G109) compared to day 21 of lactation (L21) of high litter performance sows
Abundance in Group 1: increased abundance in Day 21 of lactation (L21) of H group
Revision editor(s): Scholastica
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4H
Description: Differences in fecal microbiota in day 109 of gestation (G109) compared to day 21 of lactation (L21) of high litter performance sows
Abundance in Group 1: decreased abundance in Day 21 of lactation (L21) of H group
Revision editor(s): Scholastica
Experiment 4
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Day 109 of gestation (G109) of L group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Day 21 of lactation (L21) of L group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Day 21 of lactation (L21) of low litter performance sows group
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-7-15
Source: Figure 4K
Description: Differences in fecal microbiota in day 109 of gestation (G109) compared to day 21 of lactation (L21) of low litter performance sows
Abundance in Group 1: decreased abundance in Day 21 of lactation (L21) of L group
Revision editor(s): Scholastica
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