Association of the Cervical Microbiota With Pregnancy Outcome in a Subfertile Population Undergoing In Vitro Fertilization: A Case-Control Study/Experiment 1
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Cervical mucus Cervix mucus,Cervical mucus,cervical mucus
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Female infertility female infertility,female reproductive system infertility,female reproductive system infertility disorder,female sterility,female sub-fertility,female subfertility,infertility disorder of female reproductive system,postpartum sterility,sterility, female,sterility, postpartum,sub fertility, female,sub-fertility, female,subfertility, female,Female infertility
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-pregnant (FN)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Clinical pregnancy (FP)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients whom achieved clinical pregnancy
- Group 0 sample size Number of subjects in the control (unexposed) group
- 25
- Group 1 sample size Number of subjects in the case (exposed) group
- 26
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Source: FIG 4 (A)
Description: FIGURE 4 Shows the differential abundance and association analysis among the cervical microbiota of the fresh pregnancy group and the non-pregnancy group. (A) LEfSe analysis chart: The LDA score of the genera which showed differentially abundance between pregnant and non-pregnant women in fresh IVF-ET cycle.
Abundance in Group 1: decreased abundance in Clinical pregnancy (FP)
Revision editor(s): Rahila
Signature 2
Source: FIG 4 (A)
Description: FIGURE 4 Shows the differential abundance and association analysis among the cervical microbiota of the fresh pregnancy group and the non-pregnancy group. (A) LEfSe analysis chart: The LDA score of the genera which showed differentially abundance between pregnant and non-pregnant women in fresh IVF-ET cycle.
Abundance in Group 1: increased abundance in Clinical pregnancy (FP)
Revision editor(s): Rahila
