Social isolation induces intestinal barrier disorder and imbalances gut microbiota in mice/Experiment 2
From BugSigDB
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Social deprivation Social deprivation,social deprivation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal Control NCc (15-week normal control group)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Socially Isolated SIc (15-week socially isolated group)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individually housed mice without any sensory communication with other mice for 15 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Source: Figure 9B
Description: Differentially abundant bacterial in Normal control (NCc) and Socially isolated (SIc) mice housed for 15 weeks
Abundance in Group 1: increased abundance in Socially Isolated SIc (15-week socially isolated group)
Revision editor(s): Barrakat
Signature 2
Source: Figure 9B
Description: Differentially abundant bacterial taxa in Normal control (NCc) and Socially isolated (SIc) mice housed for 15 weeks
Abundance in Group 1: decreased abundance in Socially Isolated SIc (15-week socially isolated group)
Revision editor(s): Barrakat
