Guts within guts: the microbiome of the intestinal helminth parasite Ascaris suum is derived but distinct from its host/Experiment 6

From BugSigDB


Needs review

Curated date: 2024/04/12

Curator: Barrakat

Revision editor(s): Barrakat

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Jejunum Intestinum jejunum,Mid-intestine,Middle intestine,Jejunum,jejunum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non infected Pig Host Jejunum
Group 1 name Corresponds to the case (exposed) group for case-control studies
Ascaris suum Microbiome
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Microbiome of intestines harvested from Adult Ascaris worms that were harvested from host pig's gut
Group 0 sample size Number of subjects in the control (unexposed) group
4

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased