Guts within guts: the microbiome of the intestinal helminth parasite Ascaris suum is derived but distinct from its host/Experiment 8

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2024-7-15

Curated date: 2024/04/12

Curator: Barrakat

Revision editor(s): Barrakat, Scholastica

Subjects

Location of subjects
Germany
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Jejunum Intestinum jejunum,Mid-intestine,Middle intestine,Jejunum,jejunum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Ascaris summ (Males)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Ascaris suum (Females)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Microbiome of intestines harvested from female adult Ascaris worms that were harvested from host's (pig) gut

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-15

Curated date: 2024/04/12

Curator: Barrakat

Revision editor(s): Barrakat, Scholastica

Source: Figure 4D, Table S7

Description: Differentially abundant bacterial taxa in male versus female worms (Ascaris suum) present in German Landrace pig gut.

Abundance in Group 1: increased abundance in Ascaris suum (Females)

NCBI Quality ControlLinks
Clostridium
Mitsuokella jalaludinii
Terrisporobacter
Prevotella

Revision editor(s): Barrakat, Scholastica

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-7-15

Curated date: 2024/04/12

Curator: Barrakat

Revision editor(s): Barrakat, Scholastica

Source: Figure 4D, Table S7

Description: Differentially abundant bacterial taxa in male versus female worms (Ascaris suum) present in German Landrace pig gut.

Abundance in Group 1: decreased abundance in Ascaris suum (Females)

NCBI Quality ControlLinks
Alloprevotella
Dialister
Fibrobacter
Prevotella
Roseburia
Ruminococcus
Streptococcus porcorum
Subdoligranulum
prevotellaceae NK3B31 groupprevotellaceae NK3B31 group
Clostridium

Revision editor(s): Barrakat, Scholastica