Identification of Gut Microbiome and Metabolites Associated with Acute Diarrhea in Cats
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Huasong Bai, Tong Liu, Songjun Wang, Wenhui Gong, Liya Shen, Song Zhang, Zhanzhong Wang
Journal
Microbiology spectrum
Year
2023
Pages:
First page:
Keywords:
16srrnasequencing, acute diarrhea, gut micro biome, metagenomic sequencing
Changes in diet and environment can lead to acute diarrhea in companion animals, but the composition and interactions of the gut microbiome during acute diarrhea remain unclear. In this multicenter case-control study, we investigated the relationship between intestinal flora and acute diarrhea in two breeds of cats. Acutely diarrheic American Shorthair (MD, n = 12) and British Shorthair (BD, n = 12) and healthy American Shorthair (MH, n = 12) and British Shorthair (BH, n = 12) cats were recruited. Gut microbial 16S rRNA sequencing, metagenomic sequencing, and untargeted metabolomic analysis were performed. We observed significant differences in beta-diversity (Adonis, P < 0.05) across breeds and disease state cohorts. Profound differences in gut microbial structure and function were found between the two cat breeds. In comparison to healthy British Shorthair cats, Prevotella, Providencia, and Sutterella were enriched while Blautia, Peptoclostridium, and Tyzzerella were reduced in American Shorthair cats. In the case-control cohort, cats with acute diarrhea exhibited an increased abundance of Bacteroidota, Prevotella, and Prevotella copri and a decreased abundance of Bacilli, Erysipelotrichales, and Erysipelatoclostridiaceae (both MD and BD cats, P < 0.05). Metabolomic analysis identified significant changes in the BD intestine, affecting 45 metabolic pathways. Moreover, using a random forest classifier, we successfully predicted the occurrence of acute diarrhea with an area under the curve of 0.95. Our findings indicate a distinct gut microbiome profile that is associated with the presence of acute diarrhea in cats. However, further investigations using larger cohorts of cats with diverse conditions are required to validate and extend these findings.
Experiment 1
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Felis catus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Diarrhea Diarrhea,Diarrhoea,Watery stool,diarrhea
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy American Shorthair
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Acutely diarrheic American Shorthair
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Acutely diarrheic American Shorthair are cats with acute diarrhea condition
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 7days
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
- Matched on Factors on which subjects have been matched on in a case-control study
- health
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Needs review
Source: Figure 3, Supplemental table S3
Description: Species analysis of differences based on breeds between healthy and diarrheic cats
Abundance in Group 1: increased abundance in Acutely diarrheic American Shorthair
| NCBI | Quality Control | Links |
|---|---|---|
| Muribaculaceae | ||
| Segatella copri |
Revision editor(s): Imaspecial
Signature 2
Needs review
Source: Figure 3, Supplemental table S3
Description: Analysis of differences based on breeds between healthy and diarrheic cats using LEfSe
Abundance in Group 1: decreased abundance in Acutely diarrheic American Shorthair
| NCBI | Quality Control | Links |
|---|---|---|
| Solobacterium | ||
| Catenibacterium | ||
| Tyzzerella | ||
| Dialister |
Revision editor(s): Imaspecial
Experiment 2
Needs review
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- healthy British Shorthair
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Acutely diarrheic British shorthair
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Acutely diarrheic British shorthair are cats with acute diarrhea
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Needs review
Source: Figure 3
Description: Analysis of differences based on breeds between healthy and diarrheic cats using LEfSe
Abundance in Group 1: increased abundance in Acutely diarrheic British shorthair
| NCBI | Quality Control | Links |
|---|---|---|
| Segatella copri |
Revision editor(s): Imaspecial
Signature 2
Needs review
Source: Figure 3
Description: Analysis of differences based on breeds between healthy and diarrheic cats using LEfSe
Abundance in Group 1: decreased abundance in Acutely diarrheic British shorthair
| NCBI | Quality Control | Links |
|---|---|---|
| Thomasclavelia spiroformis | ||
| Parolsenella | ||
| Collinsella tanakaei |
Revision editor(s): Imaspecial
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