Microbial Diversity and Composition in Six Different Gastrointestinal Sites among Participants Undergoing Upper Gastrointestinal Endoscopy in Henan, China

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Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Li M, Shao D, Zhou J, Gu J, Qin J, Li X, Hao C, Wei W
Journal
Microbiology spectrum
Year
2022
Keywords:
Helicobacter pylori, gastric cardia, gastric juice, gastric pH, gastrointestinal tract, microbiota
The objective of this study was to describe and compare the dynamic microbiota characteristics in the gastrointestinal (GI) tract in Chinese participants via high-throughput sequencing techniques. The study collected saliva, esophageal swab, cardia biopsy, noncardia biopsy, gastric juice, and fecal specimens from 40 participants who underwent upper GI tract cancer screening in Linzhou (Henan, China) in August 2019. The V4 region of 16S rRNA genes was amplified and sequenced using the Illumina MiniSeq platform. The observed amplicon sequence variants (ASVs) gradually decreased from saliva to esophageal swab, cardia biopsy, noncardia biopsy, and gastric juice specimens and then increased from gastric juice to fecal specimens (P < 0.05). Each GI site had its own microbial characteristics that overlapped those of adjacent sites. Characteristic genera for each site were as follows: Neisseria and Prevotella in saliva, Streptococcus and Haemophilus in the esophagus, Helicobacter in the noncardia, Pseudomonas in gastric juice, Faecalibacterium, Roseburia, and Blautia in feces, and Weissella in the cardia. Helicobacter pylori-positive participants had decreased observed ASVs (cardia, P < 0.01; noncardia, P < 0.001) and Shannon index values (cardia, P < 0.001; noncardia, P < 0.001) compared with H. pylori-negative participants both in cardia and noncardia specimens. H. pylori infection played a more important role in the microbial composition of noncardia than of cardia specimens. In gastric juice, the gastric pH and H. pylori infection had similar additive effects on the microbial diversity and composition. These results show that each GI site has its own microbial characteristics that overlap those of adjacent sites and that differences and commonalities between and within microbial compositions coexist, providing essential foundations for the continuing exploration of disease-associated microbiota. IMPORTANCE Upper gastrointestinal (UGI) tract cancer is one of the most common cancers worldwide, while limited attention has been paid to the UGI microbiota. Microbial biomarkers, such as Fusobacteria nucleatum and Helicobacter pylori, bring new ideas for early detection of UGI tract cancer, which may be a highly feasible method to reduce its disease burden. This study revealed that each gastrointestinal site had its own microbial characteristics that overlapped those of adjacent sites. There were significant differences between the microbial compositions of the UGI sites and feces. Helicobacter pylori played a more significant role in the microbial composition of the noncardia stomach than in that of the cardia. Gastric pH and Helicobacter pylori had similar additive effects on the microbial diversity of gastric juice. These findings played a key role in delineating the microbiology spectrum of the gastrointestinal tract and provided baseline information for future microbial exploration covering etiology, primary screening, treatment, outcome, and health care products.

Experiment 1


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva , Esophagus , Gastric juice , Stomach , Feces Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva,Gullet,Oesophagus,Esophagus,esophagus,Stomach secretion,Succus gastricus,Gastric juice,gastric juice,Anterior intestine,Gaster,Mesenteron,Stomach chamber,Ventriculus,Stomach,stomach,Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Upper digestive tract disease disease of upper digestive tract,disease or disorder of upper digestive tract,disorder of upper digestive tract,disorder of upper gastrointestinal tract,upper digestive tract disease or disorder,upper gastrointestinal tract disease,Upper digestive tract disease,upper digestive tract disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Esophageal swab, cardia biopsy, noncardia biopsy, gastric juice, and fecal samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Saliva samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Saliva samples from participants undergoing upper gastrointestinal endoscopy.
Group 0 sample size Number of subjects in the control (unexposed) group
200
Group 1 sample size Number of subjects in the case (exposed) group
40
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
One month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0


Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Saliva samples

NCBI Quality ControlLinks
Neisseria
Prevotella

Revision editor(s): Aleru Divine

Experiment 2


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Saliva, cardia biopsy, noncardia biopsy, gastric juice, and fecal samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Esophageal swab samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Esophageal swab samples from participants undergoing upper gastrointestinal endoscopy.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Esophageal swab samples

NCBI Quality ControlLinks
Streptococcus
Haemophilus

Revision editor(s): Aleru Divine

Experiment 3


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Saliva, esophageal swab, noncardia biopsy, gastric juice, and fecal samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Cardia biopsy samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Cardia biopsy samples from participants undergoing upper gastrointestinal endoscopy.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Cardia biopsy samples

NCBI Quality ControlLinks
Weissella

Revision editor(s): Aleru Divine

Experiment 4


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva , Esophagus , Stomach , Gastric juice , Feces Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva,Gullet,Oesophagus,Esophagus,esophagus,Anterior intestine,Gaster,Mesenteron,Stomach chamber,Ventriculus,Stomach,stomach,Stomach secretion,Succus gastricus,Gastric juice,gastric juice,Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Saliva, esophageal swab, cardia biopsy, gastric juice, and fecal samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Noncardia biopsy samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Noncardia biopsy samples from participants undergoing upper gastrointestinal endoscopy.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Noncardia biopsy samples

NCBI Quality ControlLinks
Helicobacter

Revision editor(s): Aleru Divine

Experiment 5


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Saliva, esophageal swab, cardia biopsy, noncardia biopsy, and fecal samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Gastric juice samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Gastric juice samples from participants undergoing upper gastrointestinal endoscopy.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Gastric juice samples

NCBI Quality ControlLinks
Pseudomonas

Revision editor(s): Aleru Divine

Experiment 6


Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Saliva, esophageal swab, cardia biopsy, noncardia biopsy, and gastric juice samples
Group 1 name Corresponds to the case (exposed) group for case-control studies
Fecal samples
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Fecal samples from participants undergoing upper gastrointestinal endoscopy.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/12

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Figure 2B

Description: LEfSe results showing differentially abundant genera in different GI sites.

Abundance in Group 1: increased abundance in Fecal samples

NCBI Quality ControlLinks
Faecalibacterium
Roseburia
Blautia

Revision editor(s): Aleru Divine

Experiment 7


Needs review

Curated date: 2024/03/14

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Cardia of stomach Antrum cardiacum,Cardiac antrum,Cardiac orifice,Cardiac region,Cardial orifice,Cardial part of stomach,Gastric cardia,Pars cardiaca (gaster),Pars cardiaca gastricae,Stomach cardiac region,Cardia of stomach,cardia of stomach
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Helicobacter pylori Campylobacter pylori,Campylobacter pylori subsp. pylori,Campylobacter pyloridis,Helicobacter nemestrinae,Helicobacter pylori,helicobacter pylori
Group 0 name Corresponds to the control (unexposed) group for case-control studies
H. pylori-negative cardia group (NC)
Group 1 name Corresponds to the case (exposed) group for case-control studies
H. pylori-positive cardia group (PC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Biopsy samples from the cardia of patients with H. pylori infection.
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
20

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased

Signature 1

Needs review

Curated date: 2024/03/14

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: increased abundance in H. pylori-positive cardia group (PC)

NCBI Quality ControlLinks
Helicobacter
Pseudomonadota

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: decreased abundance in H. pylori-positive cardia group (PC)

NCBI Quality ControlLinks
Actinomycetota
Alloprevotella
Bacteroidota
Prevotella
Pseudomonas
Selenomonas

Revision editor(s): Aleru Divine

Experiment 8


Needs review

Curated date: 2024/03/14

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Stomach Anterior intestine,Gaster,Mesenteron,Stomach chamber,Ventriculus,Stomach,stomach
Group 0 name Corresponds to the control (unexposed) group for case-control studies
H. pylori-negative noncardia group (NN)
Group 1 name Corresponds to the case (exposed) group for case-control studies
H. pylori-positive noncardia group (PN)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Biopsy samples from the noncardia of patients with H. pylori infection.

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Richness Number of species
decreased

Signature 1

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: increased abundance in H. pylori-positive noncardia group (PN)

NCBI Quality ControlLinks
Pseudomonadota
Helicobacter

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: decreased abundance in H. pylori-positive noncardia group (PN)

NCBI Quality ControlLinks
Bacteroidota
Bacillota
Actinomycetota
Fusobacteriota
Prevotella
Veillonella
Neisseria
Haemophilus
Streptococcus
Pseudomonas
Brevundimonas
Alloprevotella
Fusobacterium
Porphyromonas
Bacteroides
Selenomonas
Delftia

Revision editor(s): Aleru Divine

Experiment 9


Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
H. pylori-positive noncardia group (PN)
Group 1 name Corresponds to the case (exposed) group for case-control studies
H. pylori-positive cardia group (PC)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Biopsy samples from the cardia of patients with H. pylori infection.

Lab analysis

Statistical Analysis

Signature 1

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: increased abundance in H. pylori-positive cardia group (PC)

NCBI Quality ControlLinks
Actinomycetota
Alloprevotella
Bacillota
Bacteroides
Bacteroidota
Brevundimonas
Delftia
Fusobacteriota
Fusobacterium
Haemophilus
Neisseria
Porphyromonas
Prevotella
Pseudomonas
Selenomonas
Streptococcus
Veillonella

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table 2

Description: Comparison of dominant phyla and genera in cardia and noncardia biopsy specimens

Abundance in Group 1: decreased abundance in H. pylori-positive cardia group (PC)

NCBI Quality ControlLinks
Pseudomonadota
Helicobacter

Revision editor(s): Aleru Divine

Experiment 10


Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Gastric juice Stomach secretion,Succus gastricus,Gastric juice,gastric juice
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Lower-pH (L) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Higher-pH (H) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Gastric juice samples from patients with a gastric pH level higher than 2.
Group 0 sample size Number of subjects in the control (unexposed) group
28
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Richness Number of species
increased

Signature 1

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table S4

Description: LEfSe results showing the microbial composition in gastric juice

Abundance in Group 1: increased abundance in Higher-pH (H) group

NCBI Quality ControlLinks
Prevotella
Streptococcus
Neisseria
Veillonella
Haemophilus
Rothia

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table S4

Description: LEfSe results showing the microbial composition in gastric juice

Abundance in Group 1: decreased abundance in Higher-pH (H) group

NCBI Quality ControlLinks
Pseudomonas
Acinetobacter
Herbaspirillum
Amycolatopsis

Revision editor(s): Aleru Divine

Experiment 11


Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
H. pylori-negative (N) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
H. pylori-positive (P) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Gastric juice samples from patients with H. pylori infection.
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
20

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Richness Number of species
unchanged

Signature 1

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table S4

Description: LEfSe results showing the microbial composition in gastric juice

Abundance in Group 1: increased abundance in H. pylori-positive (P) group

NCBI Quality ControlLinks
Rothia

Revision editor(s): Aleru Divine

Signature 2

Needs review

Curated date: 2024/03/15

Curator: Aleru Divine

Revision editor(s): Aleru Divine

Source: Table S4

Description: LEfSe results showing the microbial composition in gastric juice

Abundance in Group 1: decreased abundance in H. pylori-positive (P) group

NCBI Quality ControlLinks
Pseudomonas
Acinetobacter

Revision editor(s): Aleru Divine