Gut microbiota-associated taurine metabolism dysregulation in a mouse model of Parkinson's disease

Study information

Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

study design

laboratory experiment

Citation

PMID PubMed identifier for scientific articles.

37819112

DOI Digital object identifier for electronic documents.

10.1128/msphere.00431-23

URI

Authors

Cui C, Song H, Han Y, Yu H, Li H, Yang Y, Zhang B

Journal

mSphere

Year

2023

Keywords:

Lactobacillus, Parkinson's disease, metabolism, microbiota, taurine

PD is recognized as a multisystem disease concerning GI dysfunction and microbiota dysbiosis but still lacks ideal therapies. Recently, aberrant microbiota-derived metabolites are emerging as important participants in PD etiology. However, the alterations of gut microbiota community and serum untargeted metabolite profile have not been fully investigated in a PD mice model. Here, we discover sharply reduced levels of Lactobacillus and taurine in MPTP-treated mice. Moreover, Lactobacillus, Adlercreutzia, and taurine-related metabolites showed the most significant correlation with pathological and GI performance of PD mice. The abundances of microbial transporter and enzymes participating in the degeneration of taurine were disturbed in PD mice. Most importantly, taurine supplement ameliorates MPTP-induced motor deficits, DA neuron loss, and microglial activation. Our data highlight the impaired taurine-based microbiome-metabolism axis during the progression of PD and reveal a novel and previously unrecognized role of genera in modulating taurine metabolism.

Experiment 1

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/03/12

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Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Control mice

Group 1 name Corresponds to the case (exposed) group for case-control studies: MPTP-treated mice

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Mice were injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), to induce Parkinson's disease.

Group 0 sample size Number of subjects in the control (unexposed) group: 10

Group 1 sample size Number of subjects in the case (exposed) group: 9

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: decreased

Chao1 Abundance-based estimator of species richness: decreased

Richness Number of species: decreased

Signature 1

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Source: Figure 3I

Description: Cladogram of linear discriminant analysis scores for differentially abundant bacteria (phylum, class, order, and family). Only taxa meeting a linear discriminant analysis significant threshold of >2.0 are shown.

Abundance in Group 1: increased abundance in

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Actinomycetales
Actinomycetota
Bifidobacteriaceae
Bifidobacteriales
Coriobacteriaceae
Corynebacteriaceae
Corynebacterium
Nocardiaceae
Odoribacter
Rhodococcus
Rikenella
Bifidobacterium

Revision editor(s): Victoria, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Svetlana up on 2024-6-5

Curated date: 2024/03/12

Curator: Victoria

Revision editor(s): Victoria, WikiWorks

Source: Figure 3I

Description: Cladogram of linear discriminant analysis scores for differentially abundant bacteria (phylum, class, order, and family). Only taxa meeting a linear discriminant analysis significant threshold of >2.0 are shown.

Abundance in Group 1: decreased abundance in

NCBI	Quality Control	Links
Adlercreutzia
Bacteroidaceae
Bacteroidales
Bacteroidia
Bacteroidota
Butyricimonas
Coriobacteriaceae
Coriobacteriales
Coriobacteriia
Muribaculaceae
Parabacteroides
Porphyromonadaceae
Prevotella
Prevotellaceae
unclassified Paraprevotella

Revision editor(s): Victoria, WikiWorks