Analysis on the change of gut microbiota and metabolome in lung transplant patients/Experiment 2
From BugSigDB
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Lung transplantation lung transplant,transplant of lung,transplantation of lung,Lung transplantation,lung transplantation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- No lung transplant group (NT)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Lung transplant chronic rejection group (CR)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The lung transplant rejection participants were patients who survived more than 6 months after transplant and were all cases of chronic rejection.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 17
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
Signature 1
Needs review
Source: Figure 3B
Description: From the LEfSe analysis, CR group exhibited a higher abundance of Lacticaseibacillus, Enterococcus, Streptococcus and other significant taxas
Abundance in Group 1: increased abundance in Lung transplant chronic rejection group (CR)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacilli | ||
| Bacillus | ||
| Enterococcaceae | ||
| Enterococcus | ||
| Lacticaseibacillus | ||
| Lactobacillales | ||
| Streptococcus | ||
| Streptococcaceae | ||
| Enterobacteriaceae | ||
| Lactobacillaceae |
Revision editor(s): Glorious, Fiddyhamma
Signature 2
Needs review
Source: Figure 3B
Description: Within the lung transplant cohort, a significant decrease in Bacteroides, Epulopiscium, Faecalibacterium, Prevotella and other significant taxas was observed.
Abundance in Group 1: decreased abundance in Lung transplant chronic rejection group (CR)
Revision editor(s): Glorious, Fiddyhamma
