Fibroblast growth factor 21 ameliorates behavior deficits in Parkinson's disease mouse model via modulating gut microbiota and metabolic homeostasis/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Svetlana up on 2024-6-27
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Clinical treatment Clinical treatment,clinical treatment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- FGF21 + MPTP mice (mice treated with Fibroblast growth factor 21 (FGF21))
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- MPTP (untreated PD mice)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) induced Parkinson's disease (PD) mice model.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2024-6-27
Source: Figure 5C, Text
Description: LEfSe analysis with LDA score representing statistical bacterial differences in colonic microbiota between the PD (positive score) and FGF21 groups (negative score).
Abundance in Group 1: increased abundance in MPTP (untreated PD mice)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacillales | ||
| Mammaliicoccus lentus | ||
| Staphylococcaceae | ||
| Staphylococcus |
Revision editor(s): Fiddyhamma
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2024-6-27
Source: Figure 5C, Text
Description: LEfSe analysis with LDA score representing statistical bacterial differences in colonic microbiota between the PD (positive score) and FGF21 groups (negative score).
Abundance in Group 1: decreased abundance in MPTP (untreated PD mice)
Revision editor(s): Fiddyhamma
