Effects of removing in-feed antibiotics and zinc oxide on the taxonomy and functionality of the microbiota in post weaning pigs/Experiment 11

From BugSigDB


Needs review

Curated date: 2024/04/27

Curator: Fiddyhamma

Revision editor(s): Fiddyhamma

Subjects

Location of subjects
Ireland
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Abnormal sample abnormal tissue sample,altered sample,Abnormal sample,abnormal sample
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Faecal_14dpw CT(no medication) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Diarrhoea_14pw CT(no medication) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Diarrhoea samples collected at day 14 post weaning from piglets not treated with medication

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Needs review

Curated date: 2024/04/27

Curator: Fiddyhamma

Revision editor(s): Fiddyhamma

Source: Fig 5D, Suppl fig 4 and text

Description: Taxa associated with each dietary treatment in the analysis of Faecal_14dpw data. Significant species are coloured according to the treatment to which they are associated to, and are annotated in the cladogram as letters, which can be identified below it.

Abundance in Group 1: decreased abundance in Diarrhoea_14pw CT(no medication) group

NCBI Quality ControlLinks
Acidaminococcus intestini
Clostridium sporogenes
Desulfovibrio fairfieldensis
Dialister massiliensis
Escherichia coli
Intestinibaculum porci
Lactobacillus delbrueckii
Megamonas hypermegale
Megasphaera elsdenii
Megasphaera hexanoica
Megasphaera stantonii
Methylomusa anaerophila
Pelosinus fermentans
Petrocella atlantisensis
Ruminococcus bicirculans (ex Liu et al. 2021)
Selenomonas ruminantium
Selenomonas sp. oral taxon 920
Selenomonas sputigena
Desulfovibrio piger
Pseudomonadota
Gammaproteobacteria
Deltaproteobacteria
Escherichia
Megasphaera
Negativicutes
Veillonellales
Veillonellaceae
Vallitaleaceae
Enterobacterales
Desulfovibrionales
Desulfovibrionaceae
Sporomusaceae
Selenomonadales
Selenomonadaceae
Enterobacteriaceae

Revision editor(s): Fiddyhamma