Role of intestinal flora in the development of nonalcoholic fatty liver disease in children/Experiment 2
From BugSigDB
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Non-alcoholic steatohepatitis NASH,nash - nonalcoholic Steatohepatitis,non alcoholic steatohepatitis,non-alcoholic steatohepatitis,nonalcoholic Steatohepatitis,nonalcoholic steatohepatitis,Non-alcoholic steatohepatitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- NAFL (Non-alcoholic fatty liver) patients
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- NASH (Non-alcoholic steatohepatitis) patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- NAFLD patients with the presence of hepatic steatosis with necroinflammation and hepatocellular injury with or without fibrosis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 5
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- WMS
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Needs review
Source: Fig 4C, Fig 4D
Description: Differential diversity and multiple bacterial species with significantly different abundance found in the intestinal flora of patients with NAFL (Non-alcoholic fatty liver) and NASH (Non-alcoholic steatohepatitis)
Abundance in Group 1: increased abundance in NASH (Non-alcoholic steatohepatitis) patients
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Bacteroides uniformis | ||
| Eubacterium ventriosum | ||
| Lachnospiraceae bacterium 7_1_58FAA | ||
| unclassified Roseburia |
Revision editor(s): Scholastica
Signature 2
Needs review
Source: Fig 4C, Fig 4D
Description: Differential diversity and multiple bacterial species with significantly different abundance found in the intestinal flora of patients with NAFL (Non-alcoholic fatty liver) and NASH (Non-alcoholic steatohepatitis)
Abundance in Group 1: decreased abundance in NASH (Non-alcoholic steatohepatitis) patients
| NCBI | Quality Control | Links |
|---|---|---|
| unclassified Peptostreptococcaceae |
Revision editor(s): Scholastica
