The efficacy of prevention for colon cancer based on the microbiota therapy and the antitumor mechanisms with intervention of dietary Lactobacillus/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2024-4-6

Curated date: 2024/03/15

Curator: Manisha28

Revision editor(s): Manisha28, Svetlana up

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Tumor control group (CK)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Living bacteria group (LB)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
BALB/c mice (male, 6 wk) purchased from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. These mice were given oral administration of living Lactobacillus bacteria.
Group 0 sample size Number of subjects in the control (unexposed) group
30
Group 1 sample size Number of subjects in the case (exposed) group
30
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
none

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2.0
Matched on Factors on which subjects have been matched on in a case-control study
age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-4-6

Curated date: 2024/03/17

Curator: Manisha28

Revision editor(s): Manisha28, Svetlana up

Source: Figure 3g

Description: Effects of administration of Lactobacillus on living bacteria group(LB). The gut microbiota with the capacity of producing antitumor compounds are increased in living bacteria group.

Abundance in Group 1: increased abundance in Living bacteria group (LB)

NCBI Quality ControlLinks
Aeromonadales
Bacillaceae
Bacillales
Bacilli
Bacillota
Clostridia
Colwelliaceae
Coprococcus
Enterobacter
Enterobacterales
Enterobacteriaceae
Escherichia
Eubacteriales
Gammaproteobacteria
Lachnospiraceae
Lactobacillaceae
Lactobacillales
Lactobacillus
Myxococcales
Oscillospira
Prevotella
Roseburia
Turicibacter
Turicibacteraceae
bacterium AF12

Revision editor(s): Manisha28, Svetlana up

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2024-4-6

Curated date: 2024/03/17

Curator: Manisha28

Revision editor(s): Manisha28, Svetlana up

Source: Figure 3g

Description: Effects of administration of Lactobacillus on living bacteria group(LB). Some gut microbiota are decreased in living bacteria group.

Abundance in Group 1: decreased abundance in Living bacteria group (LB)

NCBI Quality ControlLinks
Bacteroidales
Butyricimonas
Cyanobacteriota
Rikenellaceae
Sphingomonadaceae
Sphingomonas
Streptophyta

Revision editor(s): Manisha28, Svetlana up