Modulation of the Gut Microbiota in Memory Impairment and Alzheimer's Disease via the Inhibition of the Parasympathetic Nervous System

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Reviewed Marked as Reviewed by Svetlana up on 2024-7-1
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Park S, Wu X
Journal
International journal of molecular sciences
Year
2022
Keywords:
Alzheimer’s disease, gut dysbiosis, mild memory impairment, parasympathetic nervous system, scopolamine
The gut microbiota has been demonstrated to play a critical role in maintaining cognitive function via the gut-brain axis, which may be related to the parasympathetic nervous system (PNS). However, the exact mechanism remains to be determined. We investigated that patients with mild cognitive impairment (MCI) and Alzheimer's disease (AD) could exhibit an altered gut microbiota through the suppression of the PNS, compared to the healthy individuals, using the combined gut microbiota data from previous human studies. The hypothesis was validated in rats to suppress the PNS by scopolamine injections. The human fecal bacterial FASTA/Q files were selected and combined from four different AD studies (n = 410). All rats had a high-fat diet and treatments for six weeks. The MD rats had memory impairment by scopolamine injection (2 mg/kg body weight; MD, Control) or no memory impairment by saline injection. The scopolamine-injected rats had a donepezil intake as the positive group. In the optimal model generated from the XGboost analysis, Blautia luti, Pseudomonas mucidoiens, Escherichia marmotae, and Gemmiger formicillis showed a positive correlation with MCI while Escherichia fergusonii, Mycobacterium neglectum, and Lawsonibacter asaccharolyticus were positively correlated with AD in the participants with enterotype Bacteroides (ET-B, n = 369). The predominant bacteria in the AD group were negatively associated in the networking analysis with the bacteria in the healthy group of ET-B participants. From the animal study, the relative abundance of Bacteroides and Bilophilia was lower, and that of Escherichia, Blautia, and Clostridium was higher in the scopolamine-induced memory deficit (MD) group than in the normal group. These results suggest that MCI was associated with the PNS suppression and could progress to AD by exacerbating the gut dysbiosis. MCI increased Clostridium and Blautia, and its progression to AD elevated Escherichia and Pseudomonas. Therefore, the modulation of the PNS might be linked to an altered gut microbiota and brain function, potentially through the gut-brain axis.

Experiment 1


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Subjects

Location of subjects
China
Singapore
Turkey
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Mild cognitive impairment (MCI) and Alzheimer’s disease (AD)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with mild cognitive impairment (MCI) and Alzheimer’s disease (AD)
Group 0 sample size Number of subjects in the control (unexposed) group
125
Group 1 sample size Number of subjects in the case (exposed) group
244
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Source: Figure 3A

Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level significantly different in healthy group versus mild cognitive impairment (MCI) and Alzheimer’s disease (AD)

Abundance in Group 1: decreased abundance in Mild cognitive impairment (MCI) and Alzheimer’s disease (AD)

NCBI Quality ControlLinks
Alistipes
Bacteroides
Parabacteroides
Phocaeicola
Ruminococcus

Revision editor(s): Samreen-19, Scholastica

Experiment 2


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Alzheimer's disease [X]Dementia in Alzheimer's disease,[X]Dementia in Alzheimer's disease (disorder),AD,AD - Alzheimer's disease,Alzheimer Dementia,Alzheimer dementia,Alzheimer Dementia, Presenile,ALZHEIMER DIS,Alzheimer Disease,Alzheimer disease,Alzheimer disease, familial,Alzheimer Type Dementia,Alzheimer's,Alzheimer's Dementia,Alzheimer's dementia,Alzheimer's disease,Alzheimer's disease (disorder),Alzheimer's disease, NOS,Alzheimers,Alzheimers Dementia,Alzheimers dementia,ALZHEIMERS DIS,Alzheimers disease,DAT - Dementia Alzheimer's type,Dementia in Alzheimer's disease,Dementia in Alzheimer's disease (disorder),Dementia in Alzheimer's disease, unspecified (disorder),Dementia of the Alzheimer's type,Dementia, Alzheimer Type,Dementia, Presenile,Dementia, Presenile Alzheimer,Disease, Alzheimer,Disease, Alzheimer's,Presenile Alzheimer Dementia,sporadic Alzheimer's disease,alzheimer's disease
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy group and mild cognitive impairment (MCI)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Alzheimer’s disease (AD)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with Alzheimer’s disease (AD)
Group 0 sample size Number of subjects in the control (unexposed) group
266
Group 1 sample size Number of subjects in the case (exposed) group
103

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Source: Figure 3A

Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level significantly different in healthy group and mild cognitive impairment (MCI) versus Alzheimer’s disease (AD)

Abundance in Group 1: increased abundance in Alzheimer’s disease (AD)

NCBI Quality ControlLinks
Escherichia

Revision editor(s): Samreen-19, Scholastica

Experiment 3


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cognitive impairment Abnormality of cognition,Cognitive abnormality,Cognitive defects,Cognitive deficits,Cognitive impairment,Intellectual impairment,cognitive impairment
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy group and Alzheimer's Disease (AD)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Mild Cognitive Impairment (MCI)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with mild cognitive impairment (MCI)
Group 0 sample size Number of subjects in the control (unexposed) group
228
Group 1 sample size Number of subjects in the case (exposed) group
141

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Source: Figure 3A

Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level significantly different in healthy group and mild cognitive impairment (MCI) versus Alzheimer’s disease (AD)

Abundance in Group 1: increased abundance in Mild Cognitive Impairment (MCI)

NCBI Quality ControlLinks
Blautia
Collinsella
Faecalibacterium
Lachnospira
Streptococcus
Thomasclavelia

Revision editor(s): Samreen-19, Scholastica

Experiment 4


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Differences from previous experiment shown

Subjects

Location of subjects
South Korea
Host species Species from which microbiome was sampled. Contact us to have more species added.
Rattus norvegicus
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Memory impairment Memory Impairment Adverse Event,Memory impairment,memory impairment
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal health group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Positive and memory deficit (MD) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats divided into the positive group (n = 10) given intraperitoneal scopolamine plus donepezil injection and those belonging to the memory deficit (MD) group (n = 10) given intraperitoneal scopolamine injection
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
20

Lab analysis

16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina, Roche454

Statistical Analysis

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Source: Figure 6F

Description: LDA scores of the bacteria at the species level significantly different in normal health group versus positive and memory deficit (MD) group

Abundance in Group 1: decreased abundance in Positive and memory deficit (MD) group

NCBI Quality ControlLinks
Alistipes indistinctus
Alistipes onderdonkii
Bacteroides uniformis
Enterocloster aldenensis
Enterocloster lavalensis
Enterococcus casseliflavus
Faecalibacterium prausnitzii
Limosilactobacillus vaginalis
Parabacteroides gordonii
Ruminococcus gauvreauii
Staphylococcus aureus
[Clostridium] symbiosum
[Ruminococcus] torques

Revision editor(s): Samreen-19, Scholastica

Experiment 5


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal health group and memory deficit (MD) group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Positive group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats in the positive group (n = 10) given intraperitoneal scopolamine plus donepezil injection
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
10

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/03/16

Curator: Samreen-19

Revision editor(s): Samreen-19, Scholastica

Source: Figure 6F

Description: LDA scores of the bacteria at the species level significantly different in positive group versus normal health and memory deficit (MD) group

Abundance in Group 1: increased abundance in Positive group

NCBI Quality ControlLinks
Bacteroides finegoldii
Bifidobacterium longum
Bifidobacterium pseudolongum
Collinsella aerofaciens
Limosilactobacillus reuteri
Subdoligranulum variabile

Revision editor(s): Samreen-19, Scholastica

Experiment 6


Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/06/29

Curator: Scholastica

Revision editor(s): Scholastica

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal health group and positive group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Memory deficit (MD) group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Rats in the memory deficit (MD) group (n = 10) given intraperitoneal scopolamine injection

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2024-7-1

Curated date: 2024/06/30

Curator: Scholastica

Revision editor(s): Scholastica

Source: Figure 6F

Description: LDA scores of the bacteria at the species level significantly different in memory deficit (MD) group versus normal health and positive group

Abundance in Group 1: increased abundance in Memory deficit (MD) group

NCBI Quality ControlLinks
Anaerorhabdus furcosa
Bacteroides faecis
Bacteroides ovatus
Clostridium celatum
Clostridium perfringens
Enterocloster citroniae
Escherichia coli
Lactonifactor longoviformis
Limosilactobacillus mucosae
Mediterraneibacter gnavus
Oscillibacter ruminantium
Parabacteroides distasonis
Thomasclavelia ramosa
Clostridium saccharophilaClostridium saccharophila

Revision editor(s): Scholastica