Modulation of the Gut Microbiota in Memory Impairment and Alzheimer's Disease via the Inhibition of the Parasympathetic Nervous System
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Alzheimer's disease [X]Dementia in Alzheimer's disease,[X]Dementia in Alzheimer's disease (disorder),AD,AD - Alzheimer's disease,Alzheimer Dementia,Alzheimer dementia,Alzheimer Dementia, Presenile,ALZHEIMER DIS,Alzheimer Disease,Alzheimer disease,Alzheimer disease, familial,Alzheimer Type Dementia,Alzheimer's,Alzheimer's Dementia,Alzheimer's dementia,Alzheimer's disease,Alzheimer's disease (disorder),Alzheimer's disease, NOS,Alzheimers,Alzheimers Dementia,Alzheimers dementia,ALZHEIMERS DIS,Alzheimers disease,DAT - Dementia Alzheimer's type,Dementia in Alzheimer's disease,Dementia in Alzheimer's disease (disorder),Dementia in Alzheimer's disease, unspecified (disorder),Dementia of the Alzheimer's type,Dementia, Alzheimer Type,Dementia, Presenile,Dementia, Presenile Alzheimer,Disease, Alzheimer,Disease, Alzheimer's,Presenile Alzheimer Dementia,sporadic Alzheimer's disease,alzheimer's disease
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy controls (HC)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Alzheimer's Disease (AD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Data was obtained from four studies (PRJEB32675, PRJNA489760, PRJNA496408, and PRJNA734525). Group 1 refers to participants with enterotype Bacteroides (ET-B) having Alzheimer's Disease (AD).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 125
- Group 1 sample size Number of subjects in the case (exposed) group
- 103
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Figure 3A
Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level
Abundance in Group 1: increased abundance in Alzheimer's Disease (AD)
NCBI | Quality Control | Links |
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Escherichia |
Revision editor(s): Samreen-19
Signature 2
Source: Figure 3A
Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level
Abundance in Group 1: decreased abundance in Alzheimer's Disease (AD)
NCBI | Quality Control | Links |
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Alistipes | ||
Bacteroides | ||
Parabacteroides | ||
Phocaeicola | ||
Ruminococcus |
Revision editor(s): Samreen-19
Experiment 2
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Cognitive impairment Abnormality of cognition,Cognitive abnormality,Cognitive defects,Cognitive deficits,Cognitive impairment,Intellectual impairment,cognitive impairment
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mild Cognitive Impairment (MCI)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Data was obtained from four studies (PRJEB32675, PRJNA489760, PRJNA496408, and PRJNA734525). Group 1 refers to participants with enterotype Bacteroides (ET-B) having Mild Cognitive Impairment (MCI).
- Group 1 sample size Number of subjects in the case (exposed) group
- 141
Lab analysis
Statistical Analysis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Figure 3A
Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level
Abundance in Group 1: increased abundance in Mild Cognitive Impairment (MCI)
NCBI | Quality Control | Links |
---|---|---|
Blautia | ||
Collinsella | ||
Faecalibacterium | ||
Streptococcus | ||
Thomasclavelia |
Revision editor(s): Samreen-19
Signature 2
Source: Figure 3A
Description: Linear discriminant analysis (LDA) scores of the bacteria at the genus level
Abundance in Group 1: decreased abundance in Mild Cognitive Impairment (MCI)
NCBI | Quality Control | Links |
---|---|---|
Alistipes | ||
Bacteroides | ||
Parabacteroides | ||
Phocaeicola | ||
Ruminococcus |
Revision editor(s): Samreen-19
Experiment 3
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Alzheimer's Disease (AD)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Data was obtained from four studies (PRJEB32675, PRJNA489760, PRJNA496408, and PRJNA734525). Group 1 refers to participants with enterotype Bacteroides (ET-B) having Mild Cognitive Impairment (MCI)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 103
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Text (under "α-Diversity and β-Diversity in the Participants with ET-B", paragraph 2)
Description: Differential abundance between AD (ET-B) and MCI (ET-B)
Abundance in Group 1: increased abundance in Mild Cognitive Impairment (MCI)
NCBI | Quality Control | Links |
---|---|---|
Blautia | ||
Faecalibacterium |
Revision editor(s): Samreen-19
Experiment 4
Subjects
- Location of subjects
- South Korea
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Rattus norvegicus
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Memory impairment Memory Impairment Adverse Event,Memory impairment,memory impairment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Memory Deficit (MD) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Rats injected with scopolamine, thus belonging to the Memory Deficit (MD) group
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 10
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Figure 6E
Description: LDA scores of bacteria at the species level
Abundance in Group 1: increased abundance in Memory Deficit (MD) group
Revision editor(s): Samreen-19
Signature 2
Source: Figure 6E
Description: LDA scores of bacteria at the species level
Abundance in Group 1: decreased abundance in Memory Deficit (MD) group
Revision editor(s): Samreen-19
Experiment 5
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Positive group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Rats injected with scopolamine and given donepezil. Groups 1 refers to this group of rats called the positive group
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
Signature 1
Source: Figure 6F
Description: LDA scores of bacteria at the species level
Abundance in Group 1: increased abundance in Positive group
NCBI | Quality Control | Links |
---|---|---|
Bacteroides finegoldii | ||
Bifidobacterium longum | ||
Bifidobacterium pseudolongum | ||
Collinsella aerofaciens | ||
Limosilactobacillus reuteri | ||
Subdoligranulum variabile |
Revision editor(s): Samreen-19
Signature 2
Source: Figure 6E
Description: LDA scores of bacteria at the species level
Abundance in Group 1: decreased abundance in Positive group
Revision editor(s): Samreen-19