Transfer of intestinal microbiota from lean donors increases insulin sensitivity in individuals with metabolic syndrome/Experiment 4

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Reviewed Marked as Reviewed by Svetlana up on 2025-2-14

Curated date: 2025/02/04

Curator: Aleru Divine

Revision editor(s): Aleru Divine, WikiWorks

Subjects

Location of subjects

Netherlands

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Small intestine Anterior intestine,Intestinum tenue,Mid intestine,Small bowel,Small intestine,small intestine

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Response to transplant Response to transplant,response to transplant

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Autologous (6 weeks samples)

Group 1 name Corresponds to the case (exposed) group for case-control studies

Allogenic (6 weeks samples)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Small intestinal biopsy samples collected from the participants 6 weeks after allogenic lean donor gut microbiota treatment.

Group 0 sample size Number of subjects in the control (unexposed) group

9

Group 1 sample size Number of subjects in the case (exposed) group

9

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

3 months

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Human Intestinal Tract Chip

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mixed-Effects Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

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Curated date: 2025/02/04

Curator: Aleru Divine

Revision editor(s): Aleru Divine, WikiWorks

Source: Supplementary Table 4

Description: Differences in gut microbiota identified between allogenic and autologous treatment groups.

Abundance in Group 1: increased abundance in Allogenic (6 weeks samples)

NCBI	Quality Control	Links
Escherichia coli
Corynebacterium sp.
Alcaligenes faecalis

Revision editor(s): Aleru Divine, WikiWorks