A preliminary study of gut dysbiosis in children with food allergy

From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-5-5
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Inoue R, Sawai T, Sawai C, Nakatani M, Romero-Pérez GA, Ozeki M, Nonomura K, Tsukahara T
Journal
Bioscience, biotechnology, and biochemistry
Year
2017
Keywords:
food allergy, gut dysbiosis, high throughput 16S rRNA gene sequencing
Gut microbiota of food allergic children was analyzed by high throughput 16S rRNA gene sequencing. Signs of gut dysbiosis, which is likely associated with gut inflammation, was observed in children with food allergies. For example, decreased abundance of genus Akkermansia but increased abundance of Veillonella was found in children with food allergy in comparison with healthy control children.

Experiment 1


Reviewed Marked as Reviewed by KateRasheed on 2025-5-5

Curated date: 2025/04/17

Curator: Shulamite

Revision editor(s): Shulamite, KateRasheed

Subjects

Location of subjects
Japan
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Food allergy Food Hypersensitivity,Food intolerance,Food allergy,food allergy
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy children
Group 1 name Corresponds to the case (exposed) group for case-control studies
Food allergic children
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Children aged 18 months to 6 years diagnosed with food allergy based on clinical symptoms and oral food challenge tests, prior to receiving any treatment.
Group 0 sample size Number of subjects in the control (unexposed) group
4
Group 1 sample size Number of subjects in the case (exposed) group
4
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 Month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-5-5

Curated date: 2025/04/17

Curator: Shulamite

Revision editor(s): Shulamite

Source: Table 2

Description: Composition of gut microbiota of healthy and food allergic children. In mean relative abundance between groups are indicated with an asterisk (*).

Abundance in Group 1: increased abundance in Food allergic children

NCBI Quality ControlLinks
Sutterella
Lachnospira
Veillonella

Revision editor(s): Shulamite

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-5-5

Curated date: 2025/04/17

Curator: Shulamite

Revision editor(s): Shulamite

Source: Table 2

Description: Composition of gut microbiota of healthy and food allergic children. In mean relative abundance between groups are indicated with an asterisk (*).

Abundance in Group 1: decreased abundance in Food allergic children

NCBI Quality ControlLinks
Akkermansia
Dorea

Revision editor(s): Shulamite