Adhesive Bifidobacterium Induced Changes in Cecal Microbiome Alleviated Constipation in Mice/Experiment 8
From BugSigDB
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Cecum mucosa Caecum mucosa,Caecum mucosa of organ,Caecum mucous membrane,Caecum organ mucosa,Cecal mucosa,Cecum mucosa of organ,Cecum mucous membrane,Cecum organ mucosa,Intestinum crassum caecum mucosa,Intestinum crassum caecum mucosa of organ,Intestinum crassum caecum mucous membrane,Intestinum crassum caecum organ mucosa,Mucosa of caecum,Mucosa of cecum,Mucosa of intestinum crassum caecum,Mucosa of organ of caecum,Mucosa of organ of cecum,Mucosa of organ of intestinum crassum caecum,Mucous membrane of caecum,Mucous membrane of cecum,Mucous membrane of intestinum crassum caecum,Organ mucosa of caecum,Organ mucosa of cecum,Organ mucosa of intestinum crassum caecum,Cecum mucosa,cecum mucosa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Control ( Constipation mice without treatment )
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB2 (Constipation mice treated with CMB with no adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 1010 CFU/mL CMB2, respectively, daily for 2 weeks
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB2 (Constipation mice treated with CMB with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Bacteroides | ||
| Unclassified S24-7Unclassified S24-7 | ||
| Lactobacillus | ||
| Clostridium | ||
| Oscillospira | ||
| Anaeroplasma | ||
| Akkermansia |
Signature 2
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB2 (Constipation mice treated with CMB with no adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Adlercreutzia | ||
| Rikenella | ||
| Odoribacter | ||
| Mucispirillum | ||
| Dehalobacterium | ||
| Coprococcus | ||
| Dorea | ||
| Ruminococcus | ||
| Desulfovibrio | ||
| unclassified Helicobacteraceae |
Revision editor(s): Joiejoie
