Adhesive Bifidobacterium Induced Changes in Cecal Microbiome Alleviated Constipation in Mice/Experiment 9
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-8
Curated date: 2025/04/08
Curator: Joiejoie
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp, Ese
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Constipation Constipation,Costiveness,Dyschezia,constipation
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Normal (Healthy mice)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CMB1 (Constipation mice treated with CMB1 with adhesion properties)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group was intragastrically administered 0.25 mL of normal saline solution containing 4 × 10^10 CFU/mL CMB1(Combined multiple strains of Bifidobacterium with adhesion property), respectively, daily for 2 weeks and loperamide (0.25 mL) intragastrically from day 15 to day 17 to induce constipation.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- log transformation
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-8
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: increased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Anaeroplasma | ||
| Bacteroides | ||
| Bifidobacterium | ||
| Coprococcus | ||
| Dehalobacterium | ||
| Desulfovibrio | ||
| Lactobacillus | ||
| unclassified Muribaculaceae |
Revision editor(s): Joiejoie, Mautin, Anne-mariesharp
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-8
Source: Fig. 6, 7 & 8
Description: Relative abundance (%) of caecal microbial taxa at the genus level in constipated mice fed CMB.
Abundance in Group 1: decreased abundance in CMB1 (Constipation mice treated with CMB1 with adhesion properties)
| NCBI | Quality Control | Links |
|---|---|---|
| Oscillospira | ||
| Rikenella | ||
| Odoribacter | ||
| Mucispirillum | ||
| unclassified Helicobacteraceae | ||
| Akkermansia | ||
| unclassified Lachnospiraceae |
Revision editor(s): Joiejoie, Anne-mariesharp
