Dietary Supplementation With Leucine or in Combination With Arginine Decreases Body Fat Weight and Alters Gut Microbiota Composition in Finishing Pigs/Experiment 3

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Reviewed Marked as Reviewed by KateRasheed on 2025-6-6

Curated date: 2025/05/06

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Sus scrofa domesticus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Colon Hindgut,Large bowel,Posterior intestine,Colon,colon
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Diet measurement Diet measurement,diet measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Leu (Leucine), Leu_Glu (1.00% Leucine + 1.00% Glutamic acid) and Leu_Arg (1.00% Leucine + 1.00% Arginine)
Group 1 name Corresponds to the case (exposed) group for case-control studies
BD (Basal diet)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
The pigs in the control group were fed a basal diet supplemented with 2.05% alanine (isonitrogenous control, BD group)
Group 0 sample size Number of subjects in the control (unexposed) group
36
Group 1 sample size Number of subjects in the case (exposed) group
12

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2


Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-6

Curated date: 2025/05/06

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 8C

Description: Linear discriminant analysis (LDA) scores for the enriched microbiota among the four groups

Abundance in Group 1: increased abundance in BD (Basal diet)

NCBI Quality ControlLinks
Flavobacterium
Ruminiclostridium_6Ruminiclostridium_6
Clostridiales vadinBB60 groupClostridiales vadinBB60 group
Anaerosporobacter

Revision editor(s): Tosin