Widespread protein lysine acetylation in gut microbiome and its alterations in patients with Crohn's disease/Experiment 4
Curated date: 2023/11/24
Curator: Folakunmi
Revision editor(s): WikiWorks, Folakunmi, MyleeeA, Aleru Divine
Subjects
- Location of subjects
- Canada
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Protein modification process protein modification,Protein modification process,protein modification process
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Metaproteome aliquot of human gut
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Lysine acetylome aliquot of human gut
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- relative abundance of kac levels (for each taxon listed in supplementary data 3) in human fecal microbiome samples lysine acetylome aliquot
- Group 0 sample size Number of subjects in the control (unexposed) group
- 209
- Group 1 sample size Number of subjects in the case (exposed) group
- 209
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Mass spectrometry
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Signature 1
Source: figure 2b
Description: Lysine acetylome-to-metaproteome ratios of quantified phyla and genera in human gut microbiome. Comparisons of the percentage of acetylation in lysine acetylomic aliquot with that in metaproteomic aliquot.
Abundance in Group 1: increased abundance in Lysine acetylome aliquot of human gut
| NCBI | Quality Control | Links |
|---|---|---|
| Fusicatenibacter | ||
| Odoribacter | ||
| Lachnoclostridium | ||
| Butyricicoccus | ||
| Bacillota |
Signature 2
Source: figure 2b
Description: Lysine acetylome-to-metaproteome ratios of quantified phyla and genera in human gut microbiome. Comparisons of the percentage of acetylation in lysine acetylomic aliquot with that in metaproteomic aliquot.
Abundance in Group 1: decreased abundance in Lysine acetylome aliquot of human gut
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Butyrivibrio | ||
| Actinomycetota | ||
| Pseudomonadota | ||
| Parasutterella | ||
| Collinsella | ||
| Chordata | ||
| Gemmiger | ||
| Homo |
