Daily full spectrum light exposure prevents food allergy-like allergic diarrhea by modulating vitamin D3 and microbiota composition
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Chen P.J., Nakano T., Lai C.Y., Chang K.C., Chen C.L., Goto S.
Journal
NPJ biofilms and microbiomes
Year
2021
The importance of sun exposure on human health is well recognized, and a recent trend in the avoidance of sun exposure has led to the risk of missing the beneficial effects such as vitamin D3 biogenesis. Vitamin D3 insufficiency is one of the risk factors for the development of food allergies (FAs), and vitamin D3 status controls gut homeostasis by modulating the microbiota. This study aimed to explore the impact of daily full spectrum light exposure (phototherapy) on the pathogenesis of FAs. Phototherapy ameliorated allergic diarrhea and improved FA-associated vitamin D3 insufficiency and dysbiosis. Fecal microbiota transplantation (FMT) of FA donor feces induced allergic diarrhea with OVA-specific IgE elevation in naïve mice. In contrast, FMT of naïve donor feces ameliorated allergic diarrhea in established FA mice, suggesting the involvement of the microbiota composition in FA. Phototherapy is an alternative approach for the prevention of FA-like allergic diarrhea through the modulation of vitamin D3 status and microbiota composition.
Experiment 1
Subjects
- Location of subjects
- Taiwan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sun exposure measurement sunlight exposure measurement,Sun exposure measurement,sun exposure measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Food allergy (FA) group
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Phototherapy (PT) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female Bagg Albino Laboratory-bred (BALB/c) mice that were sensitized twice intraperitoneally with ovalbumin (OVA, 60 μg) and aluminum hydroxide (1 mg) at a 2-week interval, followed by 15 repeated oral OVA challenges (15 mg per dose, every 2–3 days) to induce food allergy, while being exposed daily to full-spectrum light (5500 K, 12 hours per day) throughout the 9-week experimental period to evaluate the effect of phototherapy on allergic responses.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Figure 6B
Description: Differences between (FA) food allergy and (PT) phototherapy groups (95% confidential interval) at the genus and species levels.
Abundance in Group 1: increased abundance in Phototherapy (PT) group
| NCBI | Quality Control | Links |
|---|---|---|
| Parabacteroides | ||
| Ruminococcus 1Ruminococcus 1 | ||
| Parabacteroides goldsteinii |
Revision editor(s): Tosin
Signature 2
Source: Figure 6B
Description: Differences between (FA) food allergy and (PT) phototherapy groups (95% confidential interval) at the genus and species levels.
Abundance in Group 1: decreased abundance in Phototherapy (PT) group
Experiment 2
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Food allergy Food Hypersensitivity,Food intolerance,Food allergy,food allergy
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Control and Phototherapy (PT) groups
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Food allergy (FA) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female Bagg Albino Laboratory-bred (BALB/c) mice sensitized twice intraperitoneally with ovalbumin (OVA, 60 μg) and aluminum hydroxide (1 mg) at a 2-week interval, followed by 15 repeated oral OVA challenges (15 mg per dose, every 2–3 days) to induce food allergy, without exposure to phototherapy.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 3
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Signature 1
Source: Supplementary Figure 5B
Description: Linear Discriminant analysis (LDA) effect size (LeFse) among control, Phototherapy (PT) and Food allergy (FA) groups
Abundance in Group 1: increased abundance in Food allergy (FA) group
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnospiraceae bacterium NK4A136 | ||
| Peptococcaceae |
Revision editor(s): Tosin
Experiment 3
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Sun exposure measurement sunlight exposure measurement,Sun exposure measurement,sun exposure measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Food allergy (FA) and Phototherapy (PT) groups
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Control group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Naïve female Bagg Albino Laboratory-bred (BALB/c) mice that were not immunized with ovalbumin and instead received phosphate-buffered saline (PBS) intragastrically, serving as a healthy non-allergic control.
Lab analysis
Statistical Analysis
Signature 1
Source: Supplementary Figure 5B
Description: Linear discriminant analysis (LDA) effect size (LeFse) among control, Phototherapy (PT) and Food allergy (FA) groups.
Abundance in Group 1: increased abundance in Control group
| NCBI | Quality Control | Links |
|---|---|---|
| Lachnospiraceae_UCG_006Lachnospiraceae_UCG_006 |
Experiment 4
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Combination of Control and Food allergy (FA) groups
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Phototherapy (PT) group
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Female Bagg Albino Laboratory-bred (BALB/c) mice that were sensitized twice intraperitoneally with ovalbumin (OVA, 60 μg) and aluminum hydroxide (1 mg) at a 2-week interval, followed by 15 repeated oral OVA challenges (15 mg per dose, every 2-3 days) to induce food allergy, while being exposed daily to full-spectrum light (5500 K, 12 hours per day) throughout the 9-week experimental period to evaluate the effect of phototherapy on allergic responses.
Lab analysis
Statistical Analysis
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