Characteristics of the vaginal microbiome in women with premature ovarian insufficiency/Experiment 1
From BugSigDB
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Distal oviductal region,Distal portion of oviduct,Vaginae,Vagina,vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Primary ovarian insufficiency female hypergonadotropic hypogonadism,hypergonadotrophic ovarian failure,hypergonadotropic hypogonadism,hypergonadotropic hypogonadism (female),premature menopause,premature ovarian failure,premature ovarian insufficiency,primary female hypogonadism,primary ovarian failure,primary ovarian insufficiency,resistant ovary syndrome,Primary ovarian insufficiency
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Primary ovarian insufficiency (POI)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Twenty-eight women with spontaneous Primary ovarian insufficiency (POI).
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 28
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Three months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Needs review
Source: Figure 2C
Description: Microbes that significantly differed between the POI and control groups.
Abundance in Group 1: increased abundance in Primary ovarian insufficiency (POI)
| NCBI | Quality Control | Links |
|---|---|---|
| Streptococcus |
Revision editor(s): Aleru Divine
Signature 2
Needs review
Source: Figure 2C
Description: Microbes that significantly differed between the POI and control groups.
Abundance in Group 1: decreased abundance in Primary ovarian insufficiency (POI)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Brevundimonas | ||
| Odoribacter |
Revision editor(s): Aleru Divine
