Characteristics of the Intestinal Microorganisms in Middle-Aged and Elderly Patients: Effects of Smoking/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/04/01

Curator: Joiejoie

Revision editor(s): Joiejoie, Montana-D

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking status measurement Smoking status measurement,smoking status measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non- smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Current Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients who smoke
Group 0 sample size Number of subjects in the control (unexposed) group
114
Group 1 sample size Number of subjects in the case (exposed) group
74
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
3 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
4

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/04/03

Curator: Montana-D

Revision editor(s): Montana-D, Joiejoie

Source: Figure 6B

Description: Comparison of intestinal microbiota composition between smokers and non-smokers.

Abundance in Group 1: increased abundance in Current Smokers

NCBI Quality ControlLinks
Paraprevotella
Megamonas
Sutterella
Alcaligenaceae
Burkholderia
Burkholderiaceae
Shigella
P_75_a5P_75_a5
ParaprevotellaceaeParaprevotellaceae

Revision editor(s): Montana-D, Joiejoie

Signature 2

Reviewed Marked as Reviewed by Svetlana up on 2025-4-7

Curated date: 2025/04/03

Curator: Montana-D

Revision editor(s): Montana-D, Joiejoie

Source: Figure 6B

Description: Comparison of intestinal microbiota composition between smokers and non-smokers.

Abundance in Group 1: decreased abundance in Current Smokers

NCBI Quality ControlLinks
Bifidobacteriaceae
Bifidobacteriales
Bifidobacterium
Coprobacillus
Microbacteriaceae
Campylobacterales

Revision editor(s): Montana-D, Joiejoie