Higher levels of Bifidobacteria and tumor necrosis factor in children with drug-resistant epilepsy are associated with anti-seizure response to the ketogenic diet
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- Sweden
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Response to ketogenic diet Response to ketogenic diet,response to ketogenic diet
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Responders before ketogenic diet (R_Ep1)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Responders during ketogenic diet (R_Ep2)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of children with ≥50% seizure reduction during ketogenic diet treatment.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 27
- Group 1 sample size Number of subjects in the case (exposed) group
- 27
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- PCR
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Welch's T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Figure 4C
Description: Fecal microbiome of R_Ep2 group compared to R_Ep1 group
Abundance in Group 1: decreased abundance in Responders during ketogenic diet (R_Ep2)
Revision editor(s): Kavyaayala, WikiWorks
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Decreasing correlation in non-responders before ketogenic diet (NR_Ep1)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Increasing correlation in non-responders before ketogenic diet (NR_Ep1)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of children with a < 50% reduction before ketogenic diet treatment.
Signature 1
Source: Figure 4B
Description: Spearman's correlation of members of the B. longum cluster.
Abundance in Group 1: decreased abundance in Increasing correlation in non-responders before ketogenic diet (NR_Ep1)
Revision editor(s): Victoria
Experiment 3
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Decreasing correlation in non-responders during ketogenic diet (NR_Ep2)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Increasing correlation in non-responders during ketogenic diet (NR_Ep2)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of children with a <50% seizure reduction during ketogenic diet treatment.
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 4B
Description: Spearman's correlation of members of the B. longum cluster.
Abundance in Group 1: decreased abundance in Increasing correlation in non-responders during ketogenic diet (NR_Ep2)
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium longum subsp. infantis |
Revision editor(s): Victoria
Experiment 4
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-responders before ketogenic diet (NR_Ep1)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Responders before ketogenic diet (R_Ep1)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of children with ≥50% seizure reduction before ketogenic diet treatment.
Signature 1
Source: Figure 4C
Description: Fecal microbiome of R_Ep1 group compared to NR_Ep1 group
Abundance in Group 1: increased abundance in Responders before ketogenic diet (R_Ep1)
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium breve JCM 7019 | ||
Bifidobacterium longum DJO10A | ||
Bifidobacterium longum subsp. infantis | ||
Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088 |
Revision editor(s): Kavyaayala, WikiWorks
Experiment 5
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Decreasing correlation in responders during ketogenic diet (R_Ep2)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Increasing correlation in responders during ketogenic diet (R_Ep2)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- This group consists of children with a ≥50% seizure reduction during ketogenic diet treatment.
Signature 1
Source: Figure 4B
Description: Spearman's correlation of members of the B. longum cluster.
Abundance in Group 1: decreased abundance in Increasing correlation in responders during ketogenic diet (R_Ep2)
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium longum subsp. infantis | ||
Bifidobacterium longum subsp. longum JDM301 |
Revision editor(s): Victoria