Assessing the Tsetse Fly Microbiome Composition and the Potential Association of Some Bacteria Taxa with Trypanosome Establishment/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Subjects
- Location of subjects
- Cameroon
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Glossina palpalis palpalis
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Insect head , Body proper Insect head,insect head,Body,Whole body,Body proper,body proper
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Disease staging disease_staging,Disease staging,disease staging
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-mature infection
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mature infection
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Tsetse flies with mature infection with trypanosomes.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
Signature 1
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 3
Description: Operational Taxonomic Units (in the genus level) displaying a significant difference in abundance between tsetse flies with non-mature vs. mature infection with trypanosomes.
Abundance in Group 1: increased abundance in Mature infection
| NCBI | Quality Control | Links |
|---|---|---|
| Bacillus | ||
| Enhydrobacter |
Revision editor(s): Montana-D
Signature 2
Reviewed Marked as Reviewed by KateRasheed on 2025-7-7
Source: Table 3
Description: Operational Taxonomic Units (at the genus level) displaying a significant difference in abundance between tsetse flies with non-mature vs. mature infection with trypanosomes.
Abundance in Group 1: decreased abundance in Mature infection
| NCBI | Quality Control | Links |
|---|---|---|
| Ralstonia | ||
| Serratia | ||
| Staphylococcus |
Revision editor(s): Montana-D
