Cannabis sativa L. alleviates loperamide-induced constipation by modulating the composition of gut microbiota in mice/Experiment 3

From BugSigDB


Needs review

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Traditional Chinese medicine type TCM type,Traditional Chinese medicine type,traditional Chinese medicine type
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Combination of Lop (Loperamide hydrochloride-induced constipation model) and Lop +WESCL (Loperamide hydrochloride-induced constipation model + water extract of Cannabis sativa L.)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Con (blank)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Mice in the Con group were injected with the same volume of saline
Group 0 sample size Number of subjects in the control (unexposed) group
16
Group 1 sample size Number of subjects in the case (exposed) group
8

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
3


Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-20

Curated date: 2025/04/07

Curator: Tosin

Revision editor(s): Tosin

Source: Figure 6A and 6B

Description: Identification of most characteristic taxa among experimental groups by linear discriminant analysis (LDA) effect size (LEfSe).

Abundance in Group 1: increased abundance in Con (blank)

NCBI Quality ControlLinks
Alistipes
Anaeroplasma
Bacteroides
Bilophila
Candidatus Saccharimonas
Intestinimonas
Lachnoclostridium
Muribaculaceae
Clostridia vadinBB60 groupClostridia vadinBB60 group
Lachnospiraceae UCG 004Lachnospiraceae UCG 004
Lachnospiraceae ASF356Lachnospiraceae ASF356
Oscillospirales UCG 010Oscillospirales UCG 010

Revision editor(s): Tosin