Mycobiome Study Reveals Different Pathogens of Vulvovaginal Candidiasis Shape Characteristic Vaginal Bacteriome
From BugSigDB
Jump to:navigation, search
Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Zhao C, Li Y, Chen B, Yue K, Su Z, Xu J, Xue W, Zhao G, Zhang L
Journal
Microbiology spectrum
Year
2023
Keywords:
RTIs, candidiasis, fungal community, mycobiome, vulvovaginal candidiasis
Vulvovaginal candidiasis (VVC) can alter the vaginal microbiome composition and structure, and this may be correlated with its variable treatment efficacy. Integrated analysis of the mycobiome and bacteriome in VVC could facilitate accurate diagnosis of infected patients and further decipher the characterized bacteriome in different types of VVC. Our mycobiome analysis determined two common types of VVC, which were clustered into two community state types (CSTs) featured by Candida glabrata (CST I) and Candida albicans (CST II). Subsequently, we compared the vaginal bacteriome in two CSTs of VVC and two other types of reproductive tract infections (RTIs), bacterial vaginosis (BV) and Ureaplasma urealyticum (UU) infection. The vaginal bacteriome in VVC patients was between the healthy and other RTIs (BV and UU) status, it bore the greatest resemblance to that of healthy subjects. While BV and UU patients have the unique vaginal microbiota community structure, which very different with healthy women. Compared with CST II, the vaginal bacteriome of CST I VVC was characterized by Prevotella, a key signature in BV. In comparison, CST II was featured by Ureaplasma, the pathogen of UU. The findings of our study highlight the need for co-analysis and simultaneous consideration of vaginal mycobiome and bacteriome in the diagnosis and treatment of VVC to solve common clinical problems, such as unsatisfactory cure rates and recurrent symptoms. IMPORTANCE Fungi headed by C. albicans play a critical role in VVC but are not sufficient for its occurrence, indicating the involvement of other factors, such as the vaginal bacteriome. We found that different CST correspond to different bacterial composition in patients with VVC, and this could underlie the alteration of vaginal microorganism environment in VVC patients. We believe that this correlation should not be ignored, and it may be related to the unsatisfactory treatment outcomes and high recurrence rate of VVC. Here, we provided evidence for associations between vaginal bacteriome patterns and fungal infection. Screening specific biomarkers for three common RTIs paves a theoretical basis for further development of personalized precision treatment.
Experiment 1
Needs review
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Distal oviductal region,Distal portion of oviduct,Vaginae,Vagina,vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Vulvovaginal candidiasis Candidal vulvovaginitis,candidal vulvovaginitis,Candidal vulvovaginitis (disorder),Candidal vulvovaginitis NOS (disorder),Candidal: [vulvovaginitis NOS] or [cervix],candidal: [vulvovaginitis NOS] or [cervix],candidal: cervix,Candidiasis of vulva and vagina,candidiasis of vulva and vagina,Candidiasis, Vulvovaginal,Monilial vulvovaginitis,monilial vulvovaginitis,vaginal candidiasis,Vulvovaginal Candidiasis,vulvovaginal candidiasis,Vulvovaginal candidiasis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Other reproductive tracts Infection (RTI) subjects + Healthy Controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Vulvovaginal candidiasis (VVC) (I)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in this group belong to the first community state type (CST) identified from the VVC subjects.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 152
- Group 1 sample size Number of subjects in the case (exposed) group
- 9
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V2
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.0
Signature 1
Needs review
Source: Figure 4
Description: Differentially abundant taxa between three RTIs and healthy control groups
Abundance in Group 1: increased abundance in Vulvovaginal candidiasis (VVC) (I)
| NCBI | Quality Control | Links |
|---|---|---|
| Aerococcus | ||
| Bacteroides uniformis | ||
| Bifidobacterium | ||
| Bifidobacterium adolescentis |
Revision editor(s): Aleru Divine
Experiment 2
Needs review
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Vulvovaginal candidiasis (VVC) (II)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in this group belong to the second community state type (CST) identified from the VVC subjects.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 126
- Group 1 sample size Number of subjects in the case (exposed) group
- 35
Lab analysis
Statistical Analysis
Signature 1
Needs review
Source: Figure 4
Description: Differentially abundant taxa between three RTIs and healthy control groups
Abundance in Group 1: increased abundance in Vulvovaginal candidiasis (VVC) (II)
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Gardnerella |
Revision editor(s): Aleru Divine
Experiment 3
Needs review
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Bacterial vaginosis BACT VAGINITIDES,BACT VAGINITIS,BACT VAGINOSES,BACT VAGINOSIS,Bacterial Vaginitides,Bacterial Vaginitis,Bacterial Vaginoses,bacterial vaginosis,BV,Nonspecific Vaginitis,VAGINITIDES BACT,Vaginitides, Bacterial,VAGINITIS BACT,Vaginitis, Bacterial,Vaginitis, Nonspecific,VAGINOSES BACT,Vaginoses, Bacterial,VAGINOSIS BACT,Vaginosis, Bacterial,Bacterial vaginosis
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Bacterial Vaginosis (BV)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in this group were diagnosed with bacterial vaginosis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 124
- Group 1 sample size Number of subjects in the case (exposed) group
- 37
Lab analysis
Statistical Analysis
Signature 1
Needs review
Source: Figure 4
Description: Differentially abundant taxa between three RTIs and healthy control groups
Abundance in Group 1: increased abundance in Bacterial Vaginosis (BV)
Revision editor(s): Aleru Divine
Experiment 4
Needs review
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ureaplasma urealyticum urethritis infection, Ureaplasma,infections, Ureaplasma,Ureaplasma caused disease or disorder,Ureaplasma disease or disorder,Ureaplasma infection,Ureaplasma Infections,Ureaplasma infectious disease,Ureaplasma urethritis,Ureaplasma urealyticum urethritis,ureaplasma urealyticum urethritis
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Ureaplasma urealyticum (UU) infection
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in this group were women diagnosed with Ureaplasma urealyticum (UU) infection
- Group 0 sample size Number of subjects in the control (unexposed) group
- 128
- Group 1 sample size Number of subjects in the case (exposed) group
- 33
Lab analysis
Statistical Analysis
Signature 1
Needs review
Source: Figure 4
Description: Differentially abundant taxa between three RTIs and healthy control groups
Abundance in Group 1: increased abundance in Ureaplasma urealyticum (UU) infection
Revision editor(s): Aleru Divine
Experiment 5
Needs review
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Female reproductive system disease disease of female genital system,disease of female reproductive system,disease or disorder of female reproductive system,disorder of female genital system,disorder of female genital tract,disorder of female reproductive system,female reproductive disease,female reproductive system disease,female reproductive system disease or disorder,female reproductive system disorder,gynecological disease,Female reproductive system disease
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Other reproductive tracts Infection (RTI) subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Healthy Controls
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The participants in this group were healthy women
- Group 0 sample size Number of subjects in the control (unexposed) group
- 114
- Group 1 sample size Number of subjects in the case (exposed) group
- 47
Lab analysis
Statistical Analysis
Signature 1
Needs review
Source: Figure 4
Description: Differentially abundant taxa between three RTIs and healthy control groups
Abundance in Group 1: increased abundance in Healthy Controls
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Streptococcus anginosus |
Revision editor(s): Aleru Divine
Retrieved from "https://bugsigdb.org/w/index.php?title=36995230&oldid=205187"
