Multi-omics analysis of fecal microbiota transplantation's impact on functional constipation and comorbid depression and anxiety/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by KateRasheed on 2025-6-16

Curated date: 2025/04/16

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to transplant Response to transplant,response to transplant
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Fb (Patients before FMT - Fecal microbiota transplantation)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Fa (Patients After FMT - Fecal microbiota transplantation)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with functional constipation and comorbid depression/anxiety after undergoing fecal microbiota transplantation (FMT).
Group 0 sample size Number of subjects in the control (unexposed) group
4
Group 1 sample size Number of subjects in the case (exposed) group
4
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 weeks

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by KateRasheed on 2025-6-16

Curated date: 2025/04/17

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Source: Fig 2C

Description: The shift in gut microbiota before and after FMT based on metagenomic sequencing data.

Abundance in Group 1: increased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)

NCBI Quality ControlLinks
Enterococcus hermanniensis

Revision editor(s): Shulamite, Anne-mariesharp

Signature 2

Reviewed Marked as Reviewed by KateRasheed on 2025-6-16

Curated date: 2025/04/17

Curator: Shulamite

Revision editor(s): Shulamite, Anne-mariesharp

Source: Fig 2E

Description: The shift in gut microbiota before and after FMT based on metagenomic sequencing data.

Abundance in Group 1: decreased abundance in Fa (Patients After FMT - Fecal microbiota transplantation)

NCBI Quality ControlLinks
Bacteroides pyogenes

Revision editor(s): Shulamite, Anne-mariesharp