Orally administered neohesperidin attenuates MPTP-induced neurodegeneration by inhibiting inflammatory responses and regulating intestinal flora in mice
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
He D, Gao X, Wen J, Zhang Y, Yang S, Sun X, Cui M, Li Z, Fu S, Liu J, Liu D
Journal
Food & function
Year
2024
Parkinson's disease (PD), a neurodegenerative disease, is the leading cause of movement disorders. Neuroinflammation plays a critical role in PD pathogenesis. Neohesperidin (Neo), a natural flavonoid extracted from citric fruits exhibits anti-inflammatory effects. However, the effect of Neo on PD progression is unclear. This study aimed to investigate the effects of Neo on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD in mice and its underlying mechanism. Our results indicated that Neo administration ameliorated motor impairment and neural damage in MPTP-injected mice, by inhibiting neuroinflammation and regulating gut microbial imbalance. Additionally, Neo administration reduced colonic inflammation and tissue damage. Mechanistic studies revealed that Neo suppressed the MPTP-induced inflammatory response by inhibiting excessive activation of NF-κB and MAPK pathways. In summary, the present study demonstrated that Neo administration attenuates neurodegeneration in MPTP-injected mice by inhibiting inflammatory responses and regulating the gut microbial composition. This study may provide the scientific basis for the use of Neo in the treatment of PD and other related diseases.
Experiment 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Treatment Treatment,treatment
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Saline
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) group administered with MPTP 25 mg kg−1 dissolved in saline, intraperitoneally
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- unchanged
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5D, F and H
Description: Differences in the abundance of microbial taxa between Saline and MPTP groups.
Abundance in Group 1: increased abundance in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomycetota | ||
| Adlercreutzia | ||
| Allobaculum | ||
| Desulfovibrionaceae | ||
| Erysipelotrichaceae | ||
| Oscillospira | ||
| Pseudomonadota | ||
| Psychrobacter |
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5F and H
Description: Differences in the abundance of microbial taxa between Saline and MPTP groups.
Abundance in Group 1: decreased abundance in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillaceae | ||
| Lactobacillus |
Revision editor(s): MyleeeA
Experiment 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- MPTP
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- (Neo+MPTP) NM
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Donor Mice group treated with MPTP 25 mg kg−1 intraperitoneally and Neo 25 mg kg−1.
Lab analysis
Statistical Analysis
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
- Faith Phylogenetic diversity, takes into account phylogenetic distance of all taxa identified in a sample
- increased
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5D, F and H
Description: Differences in the abundance of microbial taxa between MPTP and Neo+MPTP groups.
Abundance in Group 1: increased abundance in (Neo+MPTP) NM
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidota | ||
| Lactobacillaceae | ||
| Muribaculaceae | ||
| Lactobacillus |
Revision editor(s): MyleeeA
Signature 2
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5D, F and H
Description: Differences in the abundance of microbial taxa between MPTP and Neo+MPTP groups.
Abundance in Group 1: decreased abundance in (Neo+MPTP) NM
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomycetota | ||
| Pseudomonadota | ||
| Erysipelotrichaceae | ||
| Desulfovibrionaceae | ||
| Oscillospiraceae | ||
| Psychrobacter | ||
| Adlercreutzia | ||
| Allobaculum | ||
| Oscillospira |
Revision editor(s): MyleeeA
Experiment 3
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Saline and Neo+MPTP
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- MPTP
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) group administered with MPTP 25 mg kg−1 dissolved in saline, intraperitoneally
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
Lab analysis
Statistical Analysis
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 3
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5A
Description: Differences in the abundance of microbial taxa between Saline/Neo+MPTP and MPTP.
Abundance in Group 1: increased abundance in MPTP
Revision editor(s): MyleeeA
Experiment 4
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Saline and MPTP
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Neo+MPTP (NM)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Donor Mice group treated with MPTP 25 mg kg−1 intraperitoneally and Neo 25 mg kg−1.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5A
Description: Differences in the abundance of microbial taxa between Saline/MPTP and Neo+MPTP.
Abundance in Group 1: increased abundance in Neo+MPTP (NM)
| NCBI | Quality Control | Links |
|---|---|---|
| ChloracidobacteriaChloracidobacteria | ||
| RB41RB41 | ||
| Bacteroidaceae | ||
| Bacteroides | ||
| Prevotella | ||
| Prevotellaceae |
Revision editor(s): MyleeeA
Experiment 5
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- MPTP and Neo + MPTP (NM)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Saline
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The mice in the Saline group were administered Saline orally.
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Svetlana up on 2025-3-28
Source: Figure 5A
Description: Differences in the abundance of microbial taxa between Saline and MPTP/Neo+MPTP.
Abundance in Group 1: increased abundance in Saline
Revision editor(s): MyleeeA
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